尖锐湿疣组织中MMP9表达及意义.docVIP

尖锐湿疣组织中MMP9表达及意义 　　[摘要] 目的:研究尖锐湿疣组织中MMP-9的表达及与CD105标染的疣体内微血管密度(MVD)和瘤体生物学行为的相关性。方法:运用免疫组化SP法检测MMP-9在35例尖锐湿疣组织、15例正常包皮组织中的表达及CD105标染的新生血管的密度,分析MMP-9在尖锐湿疣组织中的表达情况,进一步分析其表达与MVD值、临床病理特征及复发的关系。结果:MMP-9在尖锐湿疣组的表达水平明显高于正常包皮组(P0.01),尖锐湿疣组的MVD值高于正常包皮组(P0.01)。Spearman相关分析提示MMP-9表达水平与MVD值呈正相关。结论:MMP-9过表达可能与尖锐湿疣疣体的形成和频繁复发有关,MMP-9蛋白过度表达在尖锐湿疣疣体的血管形成中具有重要的临床意义。 　　[关键词] 尖锐湿疣;MMP-9;CD105;微血管密度;免疫组织化学 　　[中图分类号] R752.5+3[文献标识码]A [文章编号]1673-7210(2009)07(a)-019-02 　　 　　Expression and significance of MMP-9 in condyloma acuminatum tissues 　　XIE Jing, XU Qiaolian, ZHAO Meifang, LIU Xiaolan 　　(Fuyong Family Planning Service Center of Baoan District, Shenzhen City, Shenzhen 518103, China) 　　[Abstract] Objective: To study the expression of MMP-9 in condyloma acuminatum (CA) tissues and detect the correlation between CD105 stained MVD and the biological behaviors of the disease. Methods: The expression of CD105 and MMP-9 in 35 cases of CA and 15 cases of prepuces was studied by SP immunohistochemical method. The correlation between their expression levels and clinical pathological features was also analyzed. Results: The expression of MMP-9 in CA tissues was higher compared with normal prepuces (P0.01). MVD in CA group was higher than the normal prepuces (P0.01). Spearman correlation analysis suggested that the expression of MMP-9 was positively correlated with MVD. Conclusion: The overexpression of MMP-9 may be related to the occurrence and frequent recurrence of CA, and the overexpression of MMP-9 plays a key role in the angiogenesis of CA. 　　[Key words] Condyloma acuminatum; MMP-9; CD105; MVD; Immunohistochemistry 　　 　　基质金属蛋白酶类(matrix metalloproteinases,MMPs)是一种明胶酶,通过降解细胞外基质,促进恶性肿瘤的浸润和转移。基质金属蛋白酶在许多常见肿瘤中的表达多呈异常上调。近年研究表明,MMPs还可调节肿瘤血管生成,其中MMP-9被视为重要的血管生成因子[1-3]。CD105是近年来受人瞩目的新生血管标志物,它参与血管生成,在增殖内皮细胞上高表达。已有文献报道CD105抗体标记的微血管密度(micro vessel density,MVD)与多种肿瘤预后的相关性[4-7]。尖锐湿疣(condyloma acuminatum,CA)临床上具有生长快、易复发的特点,少数可在短期内因疣体过度增生而形成巨大型CA,甚至恶变,这可能与MMP-9等的异常表达有关。本研究通过免疫组化SP法检测尖锐湿疣组织中MMP-9的表