不同细胞质源甘蔗亲本与主要性状遗传表现的相关性研究-植物学专业论文.docxVIP

II II 组合的黑穗病发病率以及 135 和 138 组合的叶绿素含量在数值上有较大差异外，其余的 68 和 102、92 和 107 组合的株高、茎径、有效茎数、叶绿素含量以及 100 和 132、47 和 48 组合的叶绿素含量都差别不大。 5、对参试的 4 个不同细胞质源亲本材料组配的正反交组合后代进行 t 检验，发现 参试组合的株高、茎秆和有效茎都没有明显差异，135 和 138 组合的叶绿素含量差异达 到显著水平，515 和 481、514 和 473 的黑穗病发病率分别为 28.4 和 18.8、28.8 和 18.9， 正反交之间 t 检验证实 P=0.00636 和 P=0.00781，两对正反交组合间差异都达到极显著 水平，而且这两对正反交组合的亲本细胞质源组成相同，都是 Badila 和 Bandjarmasim Hitam，当以细胞质源同为 Bandjarmasim Hitam 的不同亲本材料 ROC22 和 ROC10 作母 本时，子代黑穗病发病率非常接近，分别为 28.4%和 28.8%，当以细胞质源为 Badila 的 崖城 58-47 为母本分别与 ROC22 和 ROC10 配成正反交组合时，子代的黑穗病发病率几 乎一致，分别为 18.8 和 18.9。 关键词：甘蔗；细胞质基因；正反交；细胞质遗传；真伪杂种 III III Related Research on the correlation between phenotypic character and different cytoplasm in Sugarcane (Saccharum officinarum L.) Cen Huafei （College of Agriculture，Guangxi University，Nanning 530004，China） Abstract Sugarcane is the most important sugar and energy crops worldwide.At present, selective breeding of sugarcane crossing hybrid combination mainly develop the potentiality of nuclear gene to sugarcane variety, but the excavation and utilization of sugarcane cytoplasm genome and relate facourable cytoplasm genome is blankness yet. So the plasmagene of sugarcane varieties all over the world limited in Bandjarmasin Hitan, Black Cheribon, Badila and Kaludia Boothan, makes some of the main is controlled by cytoplasmic genes or karyoplasm interaction effect of strain improvement blindly and inefficient. In order to demonstrate the correlation between parents of different sugarcane plasmagene and major genetic phenotypic character, karyo DNA used to authenticate descendants and differential gene expression was analysed by the means of phenotype identify and genetic polymorphisms of cpDNA and mtDNA based on the pilot studies about different cytoplasm parents and phenotype of their offspring, reciprocal cross was conducted in parents with different plasmagene. The foundation for excavating and using plasmagene in sugarcane breeding would be laied. The main results are obtained as follows: