中药复方颈康灵对颈椎病兔颈肌酶活性影响的分析-analysis of the effect of traditional chinese medicine compound jingkangling on cervical muscle enzyme activity in cervical spondylosis rabbits.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 PAGE PAGE 3 中文摘要 目的：研究长期低头位和风寒湿刺激导致的颈椎病动物模型颈部肌肉组织的 病理变化及颈康灵对其病变肌肉软组织的改善情况，探讨颈康灵治疗风寒湿痹证 型颈椎病的作用机理。 方法：将 60 只日本大耳兔随机分为正常对照组、模型对照组、颈康灵低剂 量组、颈康灵中剂量组、颈康灵高剂量组和颈复康对照组 6 组，每组 10 只，雌 雄各半。以长期低头位和风寒湿刺激的方法，对正常组以外的 50 只家兔进行 12 周造模，建立风寒湿痹证型颈椎病动物模型。然后分别以低、中、高剂量的颈康 灵灌胃给药每日 1 次，共 4 周，同时以颈复康为阳性对照。疗程结束 3 日后检测 各项指标。以生化法检测颈部肌肉组织超氧化物歧化酶(SOD)、Na+-K+-ATP 酶及 Ca2+-ATP 酶活性。 结果：模型组家兔颈部肌肉组织各酶活性均低于正常组，经统计学比较，有 显著性差异（P0.01）；颈康灵中剂量组及高剂量组均能提高模型组家兔颈部肌 肉组织各酶活性，统计学比较有显著性差异（P0.01）；颈康灵中剂量组及高剂 量组家兔颈部肌肉组织酶活性与正常组、颈复康组比较，均无显著性差异 （P0.05），颈康灵中剂量组与高剂量组之间比较则无显著性差异（P0.05）。 结论：长期低头位和风寒湿邪导致的颈椎病家兔颈部肌肉组织发生病变后， 其 SOD、Na+-K+-ATP 酶及 Ca2+-ATP 酶活性降低；颈康灵能提高颈椎病家兔颈部 病变肌肉软组织酶活性，改善颈椎病肌肉软组织的病变情况，可能与其抗氧化及 抗炎镇痛作用有关。 关键词：痹证型颈椎病 动物模型 肌肉软组织 SOD Na+-K+-ATP 酶 Ca2+-ATP 酶 中药 实验研究 ABSTRACT Purpose：Investing pathological change in musculi colli of cervical-spondylosis animal model which is induced by holding station in yielding and invading by wind-cold and dampness evil for long term. Researching the action of prepared Chinese medicine (JINGKANGLING), which has function to dispel cold evil and remove blood stasis, in improving musculi colli pathogenetic condition of cervical-spondylosis. Discussing therapeutic mechanism of JINGKANGLING in treating cervical-spondylosis which caused by wind-cold and dampness evil. Method: Dividing 60 rabbits into six groups in random. They are normal control group, model control group, low dosage group, middle dosage group ,high dosage group (using JINGKANGLING), control group using JINGGUKANG. There are 10 rabbits in each group, half female and half male. Making every group except for normal control group into model by holding station in yielding and stimulating by wind-cold and dampness evils to Set up cervical-spondylosis animal model of wind-cold and dampness syndrome.Afterwards, giving different dosages of JINGKANGLING to therapeutic groups and JINGFUKANG to control group. Detecting different indices such as enzymatic activity of SOD, Na+-K+-ATP and Ca2+-ATP by