不同运动量对血管性痴呆防治作用的研究-药理学专业论文.docxVIP

摘 摘 要 Abstract Abstract I I 摘 要 本文就不同运动量对老年血管性痴呆小鼠学习记忆障碍的防治作用以及作用机理 作了初步的研究和探讨。首先介绍了血管性痴呆的国内外临床研究和基础研究状况，分 析了运动与血管性痴呆的关系，以表明本课题的研究意义。实验部分考察了不同运动量 对记忆巩固障碍小鼠学习记忆能力、脑组织自由基、海马组织形态以及对小鼠微循环和 耐缺氧能力的影响。 方法 实验采用腹腔注射亚硝酸钠，制备学习记忆巩固障碍模型；利采用被动转笼法对昆 明种小鼠训练 6 周后，采用 Morris 水迷宫法测试小鼠学习记忆能力；比色法检测脑组 织中超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量，HE 染色观察海马组织形态学改 变；BI-2000 图像分析系统测定小鼠微循环；采用耐缺氧实验观察小鼠耐缺氧能力。 结果 结果表明，与空白对照组比较，学习记忆巩固障碍模型小鼠定向游泳实验潜伏期显 著延长（P0.05），SOD 活力显著降低，MDA 含量显著升高（P0.05），海马神经元变性、 脱落，耐缺氧存活时间显著延长；与模型对照组比较，运动训练对上述指标均有显著改 善作用。与空白对照组比较，运动训练显著改善微循环、提高耐缺氧能力。 结论 运动训练能不同程度的改善小鼠学习记忆障碍，其作用机制可能与运动能提高小鼠 清除自由基能力、改善微循环、提高耐缺氧能力有关。 关键词 不同运动量 血管性痴呆 学习记忆 自由基 海马组织形态学 微循环 耐缺氧 Abstract In this paper, we investigate the effect of exercise volume on learning and memory in senile dementia mice and determine the mechanism preliminarily. At first，to introduce vascular dementia clinical research and basic research at home and abroad, and to analyze the relationship between exercise and vascular dementia, in order to show that the research significance. Experimental effects of exercise volume on learning and memory, free radical of brain, morphology in hippocampus slices, microcirculation of mice and the impact of hypoxia tolerance. Methods The mice were ip. sodium nitrite to prepare memory consolidated disability model. After 6 weeks of exercise with the running wheels, water maze task was used to evaluate the learning and memory function of mice, Colorimetric detection of brain superoxide dismutase (SOD) activity and malondialdehyde (MDA) content, HE morphological changes in hippocampus were observed; BI-2000 image analysis system was used to evaluate the microcirculation; The hypoxia tolerance were observed on acute anoxia mice. Results The results showed that as compared with control group, the escape latency in directional swimming was significantly prolonged（P0.05）， SOD activity was decreased and the content of MDA was significantly increased（P0.05）, the hippocampus neuronal degeneration, falls off in hippocampus sli