不同虾类超氧化物歧化酶性质比较研究水生生物学专业论文.docxVIP

II II Abstract Abstract The article took Penaeus japonicus for example to detect the extract conditions of superoxide dismutase(SOD) in shrimps, and analyzed the properties of SOD in Penaeus japonicus, Procambarus clarkii, Litopenaeus vannamei, then made the comparison of isoenzymes in the three species of shrimps. SOD is the important index of oxygenic metabolism and immunity for organisms. So the research about SOD of different species of shrimps affords the basic information for the biological theories and immune mechanisms and offers the basis for healthy breed, disease prevention and cure and new pharmic exploitation of shrimps. The experiments were done to find out the appropriate conditions of SOD purified from the muscle tissue of Penaeus japonicus. When the pH of extract buffer was at 6.5, the highest activity of SOD was achieved. The heat treatment of 65℃ and 70-90% saturated ammonium sulfate could fractionated the protein. The active fraction yielded by DEAE-32 column chromatography under eluting buffer, pH7.8 was collected, SOD at higher purity was primarily obtained. PAGE identified that the protein bands had activity of SOD. Cu,Zn-SOD was made certain by the sensitivity of chloroform/ethanol. It was found that the calcium ion restrained the SOD activity of the muscle tissue from Penaeus japonicus, but it was not intense in the scale determined. The ions of copper, zinc, manganese promoted the activity in the lower concentration, but inhibited it in the higher concentration evidently. Four ions affected the SOD activity of the muscle tissue from Procambarus clarkii and Litopenaeus vannamei as in Penaeus japonicus by copper, the difference lying in the degree of the effect. SDS and EDTA controlled the SOD activity from Penaeus japonicus and Procambarus clarkii distinctly. SDS restrained the activity from Litopenaeus vannamei evidently, but EDTA promoted it. It is possible reason that the structures of the protein from different shrimps are not in the s