局部深低温对脊髓损伤大鼠神经再生和硫酸软骨素蛋白多糖mRNA表达影响.docVIP

局部深低温对脊髓损伤大鼠神经再生和硫酸软骨素蛋白多糖mRNA表达影响 　　摘要：目的 探索局部深低温对脊髓损伤大鼠硫酸软骨素蛋白多糖(chondroitin sulfate proteoglycans,CSPGs)基因表达和轴突再生的影响。方法 54只成年SD大鼠被随机分为三组：假手术组（n=6），脊髓损伤组（n=24）和脊髓损伤+低温组(n=24)，建立动脉瘤夹钳夹脊髓损伤模型，在使用硬膜外灌流装置保持干预组低温状态120min后，缓慢复温至37℃。通过Realtime-PCR 检测脊髓损伤大鼠伤后NG2 、neurocan和Brevican的表达，通过LFB染色观察大鼠脊髓脱髓鞘程度并使用大鼠后肢BBB评分评估局部低温对于脊髓损伤后运动功能恢复的影响。结果 ng2、Neurocan的mRNA表达在伤后8d左右（Brevican 14d）达到高峰，与损伤组相比，低温组在伤后8d和14d的表达水平显著降低(P0.05)，脱髓鞘及空泡化程度降低，并获得了较高的BBB评分（P0.05）。结论 局部低温能够促进脊髓损伤后运动功能的恢复，这种作用可能与局部深低温下调了硫酸软骨素蛋白多糖的基因表达有关。 　　关键词：深低温；脊髓损伤；轴突再生；硫酸软骨素蛋白多糖 　　Effects of Regional Profound Hypothermia on Axon Regeneration and Chondroitin Sulfate Proteoglycans mRNA 　　 Expression in Rats with Spinal Cord Injury 　　XU Xiao-yu1,2,CHENG Hui-lin1 　　(1.School of Medicine,Nanjing University ,Nanjing 210002,Jiangsu,China;2.Department of Neurosurgery,Nanjing General Hospital of Nanjing Military Region,Nanjing 210002,Jiangsu,China) 　　Abstract：ObjectiveTo investigate the effect of regional profound hypothermia on the mRNA expressions of chondroitin sulfate proteoglycans (CSPGs) and axon regeneration in adult rats with spinal cord injury. Methods56 rats were randomly assigned into sham-operated (n=6), spinal cord injury (SCI) (n=24), SCI+Hypothermia (n=24) groups. Spinal cord injury models were established by clamped on T10 with aneurysm clip. An epidural perfusion device was applied to maintain a steady temperature (18℃) for 120min with gradual re-warming to 37℃. The mRNA expressions of NG2, Neurocan and Brevican were tested by real-time PCR. The Luxol Fast Blue (LFB) stain were used to observe the morphology features of spinal cord. The motor function of hind limbs (BBB score) was monitored for 21 days. ResultsThe mRNA expressions of NG2, Neurocan, Brevican were significantly downregulated by hypothermia at 8D and 14D after spinal cord injury(P0.05), an alleviated demyelinating condition was observed in rats received RH, in addition, the SCI+Hypothermia group got a higher BBB score compared with the