应用组织芯片技术检测mTOR和VEGF在结肠癌中表达.docVIP

应用组织芯片技术检测mTOR和VEGF在结肠癌中表达 　　【摘 要】目的：探讨雷帕霉素靶蛋白（mTOR）与血管内皮生长因子（VEGF）在临床结肠癌患者中的表达，并针对两者与结肠癌病理参数之间存在的关联性。方法：选取72例结肠癌标本的组织芯片，采用免疫组化对mTOR以及VEGF进行检测，经过结果分析两者在结肠癌状态下与正常结肠组织中的表达，同时根据结果对mTOR、VEGF与结肠癌临床参数之间存在的关联性进行统计分析。结果：mTOR在结肠癌中的总表达率为42.5%，在正常结肠黏膜组织中的表达率为21.3%；VEGF在结肠癌中的总表达率为60.2%，在正常结肠黏膜组织中的表达率为40.4%，mTOR与VEGF在结肠癌当中的表达率明显高于在正常结肠黏膜组织中的表达率，比较差异具有统计学意义（P0.05）。mTOR表达率与性别、年龄等患者基本资料之间无明显的相关性，但mTOR与VEGF在淋巴结转移情况、肿瘤浸润深度等情况呈明显的正相关性，mTOR与VEGF之间也存在明显的正相关性。结论：mTOR与VEGF可作为结肠癌发展中的重要指标进行观察，同时mTOR与VEGF也可以作为结肠癌的靶点应用于临床当中。 　　【关键词】雷帕霉素靶蛋白；血管内皮生长因子；结肠癌；组织芯片；免疫组化 　　Abstract：objective：to investigate the rapamycin target protein（mTOR）and vascular endothelial growth factor（VEGF）expression in clinical patients with colon cancer，and for the correlation between pathological parameters with colon cancer.Methods：choose 72 cases of colon cancer specimens of tissue microarray，of the mTOR and VEGF by immunohistochemical detection，through the analysis of both in the condition of colon cancer and normal colon tissue expression，at the same time，according to the results of mTOR，correlation between VEGF and colon cancer clinical parameters were analyzed.Results：the total expression of mTOR in colon cancer at a rate of 42.5%，in the normal colonic mucosa tissue expression rate is 21.3%；Total expression of VEGF in colon cancer at a rate of 60.2%，in the normal colonic mucosa tissue expression rate is 40.4%，mTOR and the expression rate of VEGF in colorectal cancer were significantly higher than those in normal colonic mucosa tissue expression rate of comparative differences statistically significant（P 0.05）.MTOR expression rate of patients with gender，age and other basic data have no obvious correlation between，but mTOR and VEGF in lymph node metastasis，tumor infiltration depth，and so on and so forth are obvious positive correlation，also exist obvious positive correlation between mTOR and VEGF.Conclusion：the mTOR and VEGF can be used as an important index to observe in colon cancer development，at the same tim