重组融合蛋白il-18对金黄色葡萄球菌感染小鼠免疫相关炎症因子表达影响的分析-effect of recombinant fusion protein il - 18 on expression of immune-related inflammatory factors in mice infected with staphylococcus aureus.docxVIP

Ab Abstract PAGE PAGE IV ABSTRACT Objective:To observe the effects of recombinant fusion protein IL-18 on inflammatory factors, such asIL-17 、 IFN-γ 、 IL-23 、 MIP and so on, in the Staphylococcus aureus infected mice, we performed an experiment to clarify the mechanism that the recombinant fusion protein IL-18 defend Staphylococcus aureus infection in vivo defense. Methods:SPF male BLAB / c mice were randomly divided into four groups (n = 10). Group A was the control group; Group B was Staphylococcus aureus infection group; Group C was protein of Staphylococcus aureus infections in the intervention group; group D was recombinant fusion protein group. The levels of IL-4, IL-17, IL-23, IFN- γ, TNF, G-CSF, IgM in blood and BALF of mouse were observed through the ELISA method, the expression levels of MIP-1a and MIP-2β mRNA in lung tissue of mice were detected by real-time fluorescence quantitative PCR. Results:1. Serum IL-4 concentration levels in the groups B, C, and D are higher than that in group A (P 0.05). BALF IL-4 concentration levels in the groups B, C, and D are higher than that in group A (P 0.05). Among four groups, the IL-4 concentrations in BALF are higher than in serum. Serum IL-17 concentration levels in the groups B, and C are higher than that in group A (P 0.05). The serum IL-17 concentration in group D is lower than that in group A. The BALF IL-17 concentration levels in the groups B, C, and D are higher than that in group A (P 0.05). The concentration of IL-17 in BALF is higher than that in serum both group B and D. Serum IL-23 concentration levels in the groups B, C, and D are higher than that in group A(P 0.05).BALF IL-23 concentration levels in Group B, C, and D are higher than that in group A (P 0.05). The concentration of IL-23 in BALF is higher than that in serum both group A, B, and C. The concentration of IL-23 in serum is higher than that in BALF regarding group D. Serum IFN-γ concentration levels in the groups B, a