成人all中bcrabl基因和多参数免疫标记的表达及临床意义-expression of bcr abl gene and multiparameter immune markers in adult all and its clinical significance.docxVIP

他中科技 k斗持同济庆节院 呗才学位论又 患者初珍时的臼细胞数相关 .P 值均0.05 ，BCRlABL 基因、 AC m， CDJ4、白67 两两之间未发现相关生，但 BCRIABL AC 13J (或 CDJ4 ) 、 Ki67 三项中有二项以上阳性表达贝 J 与疗效之间具有相关饨 (P0.05 )。 结论本研究显示， BCR且BL 基因且 AC m、CDJ4、Ki67 $-项免 踵标记在成人意性淋巴细胞白血病中同时表达二项以上，与病人的 治疗敦果有关，可作为判断成人急性淋巴细胞白血病预后的可靠指 标p 为制定有盟的个体化治疗置赂，提供证据， 关键词成人 ALL BCRIABL AC m Ki67 CD34 疗却 Z 华中科技人学 1!iJll 且学院 舰上学位险义 The expressions ofBCRJABL bind and multi-immunophenotype and its clinical significance in adult acute lymphoblastic leukemia Master guoJmgming Tutor zou ping Abstract Objective To determine the expressions of BCR且BL bind 、CDJ4 、 Ki67 皿.d AC m in adult ALL 阻d to evaluate their impression Methods The expressions ofBCRJABL bind 、CDJ4 ，Ki67 and AC 1J1 were assayed by means of RT-PCR and flowcytome衍yin22cωes of adult ALL. The rdationship betw飞en the results and the clinical parameters was analy出d using Chi-square test 阻d logistic re岳阳SSlOn Results Af飞.er 6 weeks observation ，21 patients who received DVP or DVLP regimens chemoth巳rapy were evaluated. There were 6 patients achieved complete response(CR) 叩d 9 patients achieved partial response(PR).Patients wi 也 progressive diease were sixηle po旦tive rates of BCR且BL bind ，CDJ4 、回67 and AC Ill明 ere 27.3%(6/22) 3 华中科技大学同济庆节院 州七学位论文 54.5%(12122) ，45.5%(10122) ancl 40.5%(9川22) respectivel予 Th， expression of BCRIABL bind 、AC m and Ki67 was signi丑cantly associat后d wi川、 the response to chemotherapy 、immunotype and the tota! number of WBC in primary patients r.ωpective!y(PO .05).There V础附 significant dim盯ence betv.白n each pair of BCRI ABL bind CDJ4 ，Ki67 ancl AC lJJ.B川址le efficacy of chemotherapy was associated 飞叽th co-expre咽。n of two of three indexes ，BCRJABL bincl 、Ki67 and AC IJ](or CDJ4) Conclusion Co-expression of two of BCRJ ABL bind 、Ki67 and ACm(or CDJ4)，which is significan址y correlated to the prim 缸y efficacy of chemotherapy ，may serve as a reliable marker of progno剖S in adult ALL and be helpfu! for the setting of effective individu