聊红’槐无性繁殖技术分析-analysis on asexual propagation technology of sophora japonica.docxVIP

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聊红’槐无性繁殖技术分析-analysis on asexual propagation technology of sophora japonica.docx

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聊红’槐无性繁殖技术分析-analysis on asexual propagation technology of sophora japonica

聊城大学硕士学位论文聊城大学硕士学位论文 ii ii 生根培养基为 1/2MS + IBA 0.3 mg/L，生根率为 46.7%。将组培苗移栽入草炭+蛭石（V:V=1:1）中，每 4 d 浇一次 1/4MS 大量元素，成活率最高，为 86%。 3. 嫁接繁殖 ‘聊红’槐以春季枝接和夏秋季芽接进行嫁接繁殖，春季枝接在 4 月上中旬进行，有劈接和插皮接两种方法。夏秋季芽接在 8～9 月进行，有“T”形芽接和嵌芽接两种方法。嫁接成活率均在 70%以上，其中，春季插皮接成活率最高，为 85.4%。关键词：‘聊红’槐；硬枝扦插；组织培养；嫁接；生根率 PAGE PAGE iii ABSTRACT The hardwood cutting technique and the rooting mechanism,tissue culture and grafting technique of Sophora japonica?Liaohong? were comprehensively studied in this study. The main results were as follows: Hardwood Cutting The rooting character of cuttings was significantly influenced by exogenous hormone,concentration, medium, hardwood position, medium temperature and ways of soaking. The best root-promoting agent was ABT-1,IBA followed,and NAA was the poorest,but when comes to concentration, it was changed with exogenous hormone. The optimal rooting mediums was sand. The cuttings which were cut from the middle of and the base of one-year-old hardwood branches had higher rooting rate than the upper and the middle cuttings had the highest rooting rate of all.The rooting rate was increased by 27.5% when the medium temperature was inhanced by 8 ℃. Compared to soaking the base of the cuttings,the rooting rate was increased by 34.7% by soaking both ends at 500 mg/L NAA. The results of orthogonal test showed that the arrangement of factors influencing rooting were hardwood position, concentration, medium temperature, exogenous hormone and medium. If the middle cuttings were dealt with 300 mg/L ABT-1 and cultured in the sand at 18 ℃,the rooting rate will get up to 78.9%. The dynamic changes of nutritional substances,endogenous hormones and enzymatic defense system in cuttings were related to the rooting processes closely. The rooting rate had an extremely significantly positively correlation with dissoluble sugar content, C/N value, IAA/ABA value, PPO activity and significantly positively correlation with IAA content,POD activity but had an extremely significantly negative