独活寄生汤含药血清对大鼠退变软骨细胞Bcl―2Bax表达影响.docVIP

独活寄生汤含药血清对大鼠退变软骨细胞Bcl―2Bax表达影响 　　【摘 要】目的：探讨独活寄生汤含药血清对大鼠退变软骨细胞Bcl-2、Bax表达的影响。方法：建立体外退变软骨细胞模型，分别给予独活寄生汤含药血清（含药血清组）和空白血清（空白血清组）干预24 h、48 h、72 h，收集软骨细胞，运用RT-PCR法检测血清干预后退变软骨细胞Bcl-2、Bax mRNA表达，Western Blot法检测血清干预后退变软骨细胞Bcl-2、Bax蛋白表达。结果：独活寄生汤含药血清对Bcl-2和Bax蛋白表达的影响与其对Bcl-2和Bax mRNA的影响具有相同的趋势，随着干预时间的延长，空白血清组和含药血清组Bcl-2蛋白阳性表达率均逐渐升高，含药血清组升高更显著；Bax蛋白阳性表达率逐渐降低，含药血清组降低更明显，且各时点之间比较，差异有统计学意义（P 0.01）。结论：独活寄生汤能通过上调大鼠骨关节炎软骨细胞抑制凋亡因子Bcl-2表达，下调促凋亡因子Bax表达，抑制线粒体凋亡通路的激活，从而有效抑制软骨细胞凋亡。 　　【关键词】 骨关节炎；独活寄生汤；含药血清；软骨细胞；细胞凋亡；大鼠 　　doi：10.3969/j.issn.2095-4174.2014.12.008 　　Effects of Serum Medicated with Duhuo Jisheng Decoction on the Expression of the Degenerated Cartilage Cells Bcl-2 and Bax in Rats 　　PAN Cai-bin，WANG Wu-lian，WU Guang-wen，FU Chang-long，WENG Mei-rong，LIU Xian-xiang 　　【ABSTRACT】Objective：To observe the effects of serum medicated with Duhuo Jisheng Decoction on the expression of the degenerated cartilage cells Bcl-2 and Bax in rats.Methods：The degenerated cartilage cell models in vitro were established，which were intervened by the serum medicated with Duhuo Jisheng Decoction（the medicated serum group）and the blank serum （the blank serum group）for 24 h，48 h and 72 h，collecting chondrocytes.The RT-PCR method was used to detect the expression of degenerated cartilage cells Bcl-2 and Bax mRNA after serum intervention.The Western Blot method was used to detect the expression of degenerated cartilage cells Bcl-2 and Bax protein after serum intervention.Results：The effects of the serum medicated with Duhuo Jisheng Decoction on the expression of Bcl-2 and Bax protein had the same tendency as on the expression of Bcl-2 and Bax mRNA，.along with the extension of time，the positive expression rate of Bcl-2 protein in the two groups gradually increased，the medicated serum group being more significant than the blank serum group；while the positive expression rate of Bax protein in the two groups gradually decreased，the medicated serum group being more significant.The difference between each t