清金颗粒对耐甲氧西林金黄色葡萄球菌感染肺炎模型小鼠影响.docVIP

清金颗粒对耐甲氧西林金黄色葡萄球菌感染肺炎模型小鼠影响 　　摘要：目的 观察清金颗粒对耐甲氧西林金黄色葡萄球菌（MRSA）肺炎模型小鼠的干预效应。方法 采用菌液滴鼻法制作肺炎小鼠模型。将实验小鼠随机分为空白组、模型组、西药组和中药组，造模后第3日开始给药，西药组给予利奈唑胺灌胃，中药组给予清金颗粒灌胃，空白组和模型组给予生理盐水灌胃。造模后每日观察小鼠体质量变化及临床表现，第3、10日CT检测肺部影像学变化，HE染色观察肺组织病理变化，检测肺部载菌量。结果 造模组小鼠感染后第1日体质量显著下降，感染后第3日肺部CT示两肺充满炎性渗出物；给药后第7日中药组较模型组小鼠肺部炎症明显减轻，炎性渗出物减少，仅有散在炎性渗出物，同时肺部炎性病理表现减轻，且肺部载菌量明显降低（P0.05），但较西药组效果稍差（P0.05）。结论 清金颗粒能够显著降低小鼠肺部载菌量，减少肺组织炎细胞浸润，对MRSA感染肺炎小鼠有治疗作用。 　　关键词：清金颗粒；耐甲氧西林金黄色葡萄球菌；肺炎；小鼠 　　DOI：10.3969/j.issn.1005-5304.2018.02.013 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2018）02-0055-04 　　Effects of Qingjin Granules on Methicillin-resistant Staphylococcus Aureus Pneumonia Mice 　　ZHANG Shao-yan， ZHANG Ye-min， WU Ding-zhong， MA Zi-feng， 　　QIAN Jia-hua， ZHANG Hui-yong， LU Zhen-hui 　　Longhua Hospital Shanghai University of Traditional Chinese Medicine， Shanghai 200032， China 　　Abstract： Objective To observe the effects of Qingjin Granules on methicillin-resistant staphylococcus aureus （MRSA） pneumonia mice. Methods Pneumonia model was prepared by intranasal drip of bacterial fluid. Experimental mice were randomly divided into control group， model group， Western medicine group and TCM group. On the third day after infection， the Western medicine group was treated with linezolid， and the TCM group was given Qingjin Granules for gavage. The control and model group were given the same amount of normal saline for gavage. Body weight and clinical manifestations every day of each mouse after infected were recorded. Lung scans at day 3 and day 10 were taken； the pathological changes trough HE staining were observed； microbial load of lung tissue was detected. Results There were significant weight losses on mice except control group on the first day after infection. CT scan showed that the lung inflammation was filled with mice on the third day after infection. The lung inflammation in the TCM group was more reduced than model group， with less inflammatory exudate and only scattered inflammatory exudates. Meanwhile， pulmona