肾综合征出血热患者发热期血清酶和细胞因子水平研究.docVIP

肾综合征出血热患者发热期血清酶和细胞因子水平研究 　　[摘要] 目的 探讨肾综合征出血热发热期患者的血清酶和细胞因子含量的变化对临床辅助诊断的价值。 方法 流行性肾综合征出血热的诊断和发热期的分期依据国家卫生行业标准WS278-2008，共37例符合发热期患者作为患者组，且都是该院2013年1月―2016年12月住院患者。30名健康献血者作为对照组，其一般资料与患者组没有统计学上的差异。汉坦病毒IgM抗体检测采用金标法。两组人员血清中酶含量和白?胞介素水平分别通过贝克曼生化仪和酶联免疫法检测。 结果 37例肾综合征出血热发热期患者IgM抗体全部阳性。与对照组ALT（20±7）U/L和CK（38±11）U/L比较，发热期患者ALT（62±13）U/L和CK（210±37）U/L显著升高（P0.01），而AST、LDH和ALP两组之间差异无统计学意义。发热期患者IL-2（107.4±12.1）pg/mL、IL-6（69.5±12.6）pg/mL、IL-8（72.3±10.2）pg/mL、IL-10（30.9±5.1）pg/mL表达与对照组IL-2（50.2±7.4）pg/mL、IL-6（37.4±9.6）pg/mL、IL-8（33.6±8.5）pg/mL、IL-10（14.5±4.8）pg/mL比较差异有统计学意义（P0.01），但IL-5水平两组间差异无统计学意义。 结论 在怀疑肾综合征出血热的发热期，检测血清中ALT、CK和人白细胞介素水平具有辅助临床诊断的价值。 　　[关键词] 汉坦病毒；抗体；酶；白细胞介素 　　[中图分类号] R512 [文献标识码] A [文章编号] 1674-0742（2017）11（b）-0029-03 　　[Abstract] Objective This paper tries to investigate and its clinical value of serum enzyme and cytokine in patients with hemorrhagic fever and renal syndrome. Methods Epidemic hemorrhagic fever with hemorrhagic fever and phlegm were based on the national health industry standard WS278-2008， a total of 37 cases of patients with fever were selected as the patient group， and were treated in this hospital from January 2013 to December 2016. 30 cases of healthy blood donors were selected as the control group， and there was no statistical difference in the general information with the patient group. Hantaan virus IgM antibody detection used gold standard method. Serum levels of enzyme and interleukin were measured by Beckman biochemical and enzyme-linked immunosorbent assay （ELISA）. Results 37 acute hemorrhagic fever with renal syndrome patients were confirmed by positive of IgM antibodies. The levels of the ALT was （20±7）U/L and CK was （38±11）U/L in the control group， significantly lower than that in the patient group of （62±13）U/L and （210±37）U/L P0.01； whereas the levels of AST， LDH and ALP were not significantly different between the two groups. The levels of IL-2 was（107.4±12.1）pg/mL， IL-6 was （69.5±12.6）pg/mL， IL-8 was （72.3±10.2）pg/mL， and IL-