肿瘤相关巨噬细胞在甲状腺乳头状癌中表达及临床意义.docVIP

肿瘤相关巨噬细胞在甲状腺乳头状癌中表达及临床意义 　　[摘要] 目的 观察肿瘤相关巨噬细胞（TAMs）在甲状腺乳头状癌（PTC）的表达，探讨其在PTC侵袭转移中的作用。方法 选取我院2010年10月～2013年10月期间经病理诊断为PTC患者80例，取病理标本，采用免疫组化法检测TAMs表达，对其临床特征进行分析。 结果 TAMs在PTC中存在较高的表达（3～66/HP），高密度TAMs组肿瘤组织直径（1.6±0.1）cm明显大于低密度TAMs组（1.0±0.1）cm（P0.01）。高密度TAMs组较低密度TAMs组肿瘤浸润深度更深，且有更多的淋巴结转移，Ⅲ期以上病例数也更多。 结论 TAMs有促进PTC生长的作用，在PTC的侵袭和转移中发挥促进作用。 　　[关键词] 肿瘤相关巨噬细胞；甲状腺乳头状癌；免疫组化；侵袭转移 　　[中图分类号] R736.1 [文献标识码] A [文章编号] 1673-9701（2015）34-0011-03 　　The expression and clinical significance of tumor-associated macrophages in papillary thyroid carcinoma 　　CHENG Feng1 WANG Weibin2 ZHU Lei1 　　1.Department of General Surgery，the Central Hospital of Lishui City in Zhejiang Province， Lishui 323000， China；2.Department of Oncology Surgery，the First Affiliated Hospital to College of Medicine of Zhejiang University， Hangzhou 310003， China 　　[Abstract] Objective To investigate the expression of tumor-associated macrophages（TAMs） in human papillary thyroid carcinoma （PTC） and the role of TAMs in invasion and metastasis of PTC. Methods Eighty patients who underwent surgeries after being diagnosed with PTC from Oct 2010 to Oct 2013 were included. Their paraffin-embedded tumor tissues were investigated using immunohistochemistry （IHC） and the clinical characteristics according to TAMs density was evaluated. Results The TAM densities varied from 3-66/HP， and tumors in the high density group were larger than those in the low density group [（1.6±0.1） cm vs （1.0±0.1） cm，P0.01]. Meanwhile， in the high density group， the tumor infiltration was deeper， and more lymph node metastasis and the number of post-phase Ⅲ cases were found. Conclusion TAMs has an effect on promoting the growth of the PTC， and plays an important role in invasion and metastasis of PTC. 　　[Key words] Tumor-associated macrophages； Papillary thyroid carcinoma； Immunohistochemistry； Invasion and metastasis 　　肿瘤相关的巨噬细胞（tumor-associated macrophages，TAMs），早在上世纪80年代被描述，是一类与肿瘤相关有复杂功能的单核-巨噬细胞的总称，由外周血单核细胞在一些趋化因子和肿瘤坏死因子的作用下定向迁移至肿瘤组织中分化而成。TAMs是一