退变髓核细胞拉伸实验形态学研究.docVIP

退变髓核细胞拉伸实验形态学研究 　　【摘要】 目的：探讨退变髓核细胞拉伸实验对髓核细胞形态学的影响，为临床牵引治疗腰椎间盘突出症提供理论依据。方法：取椎间盘突出症患者术中取出的废弃髓核组织6例，将培养后的髓核细胞接种到拉伸板上，分别以5%、10%和15%拉伸幅度拉伸24 h，高倍镜下观察细胞形态学变化；腰椎间盘突出症患者使用体重20%、30%、35%牵引力的重量牵引，拍摄牵引前、牵引30 min时的腰椎侧位X线片，测量并计算各椎间隙高度的变化。结果：退变髓核细胞5%幅度拉伸24 h后髓核细胞形态轻度好转，10%幅度拉伸24 h后髓核细胞形态接近正常髓核细胞，15%幅度拉伸24 h后髓核细胞形态变差。不同组的细胞个数存在统计学差异（P5%拉伸幅度组对照组15%拉伸幅度组；腰椎间盘突出症患者牵引30 min时三组椎间隙增加幅度存在统计学差异（P0.05），表现为20%体重组30%体重组35%体重组。结论：5%拉伸幅度可促进退变髓核细胞的缓慢修复，15%拉伸幅度不利于退变髓核细胞的修复，10%拉伸幅度有利于促进退变髓核细胞的较快修复。30%体重牵引力适合于腰椎间盘突出症患者的牵引治疗。 　　【关键词】 人髓核细胞； 退变； 拉伸； 形态学 　　【Abstract】 Objective：To discuss the effect on nucleus pulposus cells morphology about the tensile experiment by using the degenerative nucleus pulposus cells.To provide the oretical basis for the traction treatment of lumbar disc prolapse.Method：The nucleus pulposus specimens were collected from patients with intervertebral disc protrusion， who were all volunteers.Nucleus pulposus cells were inoculated the cultured into the BioFlex culture plate.The morphological changes of the cells were observed at high magnification after stretched for twenty-four hours with the tensile range of 5%， 10% and 15%. The patient was given the lumber traction by using 20%， 30% and 35% of his body weight and the patient，s lumbar lateral radiographs were shot before and after traction. The changes of intervertebral space height were measured and calculated.Result：For the morphology of the degenerative nucleus pulposus cells，it improved mildly under the tensile range of 5%，it was close to normal nucleus pulposus cells under the tensile range of 10% and it deteriorated under the tensile range of 15% after twenty-four hours.There was significant difference between the number of cells（P5% tensile range groupthe control group15% tensile range group.Compared with the increasing range of intervertebral space before and after traction，there was significant difference（P 　　【Key words】 Human nucleus pulposus cells； Degeneration； Tensile； Morphological 　　脊柱退变始于椎间盘，30岁后即可发生，生物力