黎药经验方致突变试验研究.docVIP

黎药经验方致突变试验研究 　　【摘 要】 目的：考察黎药经验方的遗传毒性。方法：致突变试验采用鼠伤寒沙门菌回复突变试验（ Ames 试验）、小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸型试验等三项试验。Ames 试验：设受试物5个剂量组（即5.000、1.000、0.200、0.040、0.008mg/皿），另设未处理对照组、溶剂对照组和阳性对照组； 微核试验：小鼠50只，雌雄各半，设10、5.0、2.5 g/kg?bw 剂量组，另设溶剂对照和阳性对照组； 精子畸型率试验：昆明种小鼠雄性25只，分组同微核实验，检测精子畸形率。结果：Ames 试验中，加或不加 S9 的情况下，受试物对TA97、TA98、TA100和TA102菌株均未显示致突变作用。与溶剂对照组比较，高、中、低3个剂量的受试物组雄性、雌性小鼠微核试验、小鼠精子畸变率差异无统计学意义（P0.05）。结论：综合考虑前期试验结果及相关研究，在本次实验条件下，在一定剂量范围内，Ames 试验、 微核试验和小鼠精子畸变试验结果显示阴性，认为该黎药经验方具有遗传毒性的可能性较小。 　　【关键词】 验方；黎族；致突变；遗传毒性 　　【中图分类号】R29 【文献标志码】 A【文章编号】1007-8517（2016）24-0043-03 　　Abstract：Objective To study the mutagenesis caused by sproved recipe of the Li.Methods Ames test Test substance groups of 5.000 mg /dish，1.000，0.200，0.040 mg /dish and 0.008 mg /dish and three control groups（untreated，solvent and positive control） to detect the rechanged bacterium colonies.Micronucleus test 50 samples of the Kunming mice were randomly divided into positive control group，solvent control group and group of test substance 10.0g/kg，5.0g/kg，and 2.5g/kg.There were 10 in each group（male and female in half）.The micronucleus rate of bone marrow polychromatic erythrocyte were detected； Sperm malformation rate test 25 samples of the Kunming male mice， designed the groups as micronucleus test， were testing the rate of sperm abnormality.Results Comparing with control group，bone marrow cell micronucleus rate and sperm malformation rate of high，middle and low test substance groups of mice，what had no statistical significance（P0.05）. Compared with the untreated group，different doses of test substance Ames strains from the back variables did not change significantly （P0.05）. Conclusion Considering the preliminary test results and related research， under the conditions of this experiment and in the definite dose range，the three test results above show negative results. There is little possibility that test substance of proved recipe from the Li has mutagenesis. 　　Keywords：Proved Recipe；The Li；Mutagenesis； Genetic Toxicit