麦冬多糖MDG―1对膳食诱导肥胖模型小鼠肠道益生菌群多样性影响研究.docVIP

麦冬多糖MDG―1对膳食诱导肥胖模型小鼠肠道益生菌群多样性影响研究 　　[摘要]目的：研究麦冬多糖MDG-1对膳食诱导肥胖小鼠肠道益生菌群多样性的影响，特别关注肠道益生乳酸菌多样性的变化。方法：选取8周龄雄性C57BL/6J小鼠60只，给予高脂饲料（research diets，D12492）喂养6周及适应性灌胃2周，剔除体重低于30 g和灌胃体重降幅超过10%的动物，将诱导成功的肥胖模型小鼠按体重和血糖随机分为DIO模型组（diet-induced obesity，DIO）以及MDG-1高（300 mg?kg-1）、中（150 mg?kg-1）、低（75 mg?kg-1）剂量给药组;模型组及MDG-1不同浓度治疗组用高脂饲料喂养，对照组小鼠始终给予低脂饲料（research diets， D12450B）喂养，每组各12只。治疗组小鼠分别以不同浓度的MDG-1溶液灌胃，模型组和对照组小鼠以对照溶剂（生理盐水）灌胃，持续16周，于治疗终点收集各组小鼠直肠内容物。通过传统培养方法结合变性梯度凝胶电泳（DGGE）法对小鼠的肠道益生菌进行培养，统计菌落数量，分析模型动物口服不同浓度的MDG-1前后肠道益生菌群的变化，通过16S rDNA测序，建立系统发育树以及利用PCR-DGGE技术等分子鉴定手段确定小鼠肠道菌群变化的优势菌群。结果：不同浓度的MDG-1均可增加小鼠肠道益生菌群数量，尤其是鼠乳杆菌和台湾乳杆菌，且随浓度增加作用逐渐增强。结论：不同给药剂量的MDG-1可在一定程度上增加小鼠肠道益生菌的数量，尤其是台湾乳杆菌和鼠乳杆菌，同时改善肠道菌群多样性，促进肠道益生菌的增殖。 　　[关键词]麦冬多糖;MDG-1;肥胖;肠道菌群;多样性 　　Effect of MDG-1， a polysaccharide from Ophiopogon japonicas， 　　on diversity of lactobacillus in diet-induced obese mice 　　SHI Lin-lin， WANG Yuan， FENG Yi* 　　（Shanghai University of Traditional Chinese Medicine， Engineering Research Center of Modern Preparation 　　Technology of Traditional Chinese Medicine of Ministry of Education， Shanghai 201203， China） 　　[Abstract]For understanding the effect of MDG-1， a water-soluble β-D-fructan polysaccharide from Ophiopogon japonicas， on intestinal microecological balance， especially on the changes of lactobacillus， sixty 8-week-old male C57BL/6J mice were given a high-fat diet for six weeks and were also gavaged with saline once a day simultaneously. Then the mice which is below 30 grams or dropped more than 10% through lavage were eliminated and the rest were randomly divided into four groups： diet-induced obese （DIO） model group （n=12， gavaged with saline）， low-dose MDG-1 group （n=12， gavaged with MDG-1， 75 mg?kg-1）， medial-dose MDG-1group （n=12， gavaged with 150 mg?kg-1）， and high-dose MDG-1group （n=12， gavaged with 300 mg?kg-1） according to the weight and blood glucose; the model group and MDG-1 group were placed on a high-fat diet while the normal control group （n=12， gavaged with saline） were kept