麦冬寡糖提取纯化及抑制α―葡萄糖苷酶活性研究.docVIP

麦冬寡糖提取纯化及抑制α―葡萄糖苷酶活性研究 　　[摘要] 目的 研究麦冬中寡糖的提取分离纯化工艺，并考察其抑制α-葡萄糖苷酶活性。 方法 采用乙醇和水提取麦冬寡糖，以Sephadex G-75柱层析法进行脱色脱蛋白，利用基质辅助激光解析飞行质谱仪（MALDI-TOF-MASS）检测其分子量范围；采用来源于啤酒酵母的α-葡萄糖苷酶和来源于大鼠的具有α-葡萄糖苷酶活性的小肠绒毛提取物，考察麦冬寡糖的抑制α-葡萄糖苷酶活性。 结果 采用乙醇和水提取得到的麦冬提取液中寡糖含量为86.73%，提取率为69.4%；利用Sephadex-G75柱层析法能有效地纯化麦冬寡糖，除去色素和蛋白质分子，麦冬寡糖提取物的总寡糖含量达到97.64%，其分子量范围在500～2500之间；该麦冬寡糖对α-葡萄糖苷酶活性具有显著抑制作用。 结论 本研究提取纯化麦冬寡糖的方法是合理、可行的；麦冬寡糖具有显著抑制α-葡萄糖苷酶活性。 　　[关键词] 麦冬寡糖；提取纯化；α-葡萄糖苷酶 　　[中图分类号] R284.1 [文献标识码] A [文章编号] 1673-7210（2017）02（a）-0020-03 　　Extraction、purification and α-glucosidase inhibitory activity of oligosaccharide from Ophiopogonis japonicas 　　XU Dingzhou SU Weiwei WANG Yonggang PENG Wei WU Zhong LI Peibo 　　College of Life Sciences，Sun Yat-sen University，Guangzhou 510275， China 　　[Abstract] Objective To investigate the extraction，purification and α-glucosidase inhibitory activity of oligosaccharide from Ophiopogonis japonicas. Methods In this study，Ophiopogonis japonicas oligosaccharides were extracted with 95% alcohol and water successively， and the extract was purified by Sephadex G-75 chromatography to remove protein and pigment.The molecular weight range was determined by MALDI-TOF-MASS. The α-glucosidases of rat intestines and Saccharomyces cerevisiae were used to evaluate the inhibitory activity of Ophiopogonis japonicus oligosaccharides. Results The content of oligosaccharide extracted with 95% alcohol and water successively was 86.73%，and the extraction rate was 69.4%. After being purified by Sephadex G-75 chromatography， the protein and pigment in oligosaccharide extract was removed effectively and the content of oligosaccharide increased to 97.64%.The molecular weights were in the range of 500-2500. Furthermore，Ophiopogonis japonicas oligosaccharide could significantly inhibit the activity of α-glucosidase. Conclusion The methods of extraction and purification are technically reasonable and feasible；Ophiopogonis japonicus oligosaccharide possess significant α-glucosidase inhibitory activity.