多肽偶联的荧光硅纳米颗粒的体外癌细胞成像和靶向治疗研究-生物学专业论文.docxVIP

I I 多肽偶联的荧光硅纳米颗粒的体外癌细胞成像和靶向治疗研究 中文摘要 中文摘要 在过去的十几年中，荧光硅纳米颗粒（Silicon nanoparticles，SiNPs）以独特 的光学性质和良好的生物安全性的优势，已成为一种新型的有前途的荧光纳米探 针。精氨酸-甘氨酸-天冬氨酸（Arginine-Glycine-Aspartic acid，RGD）多肽作为 一种典型的生物功能小分子，具有比蛋白质更小的尺寸、更高的稳定性和较低的 免疫原性，其拥有巨大的潜力作为癌细胞的靶向配体。 在本文中，我们介绍了一种多肽偶联的荧光硅纳米颗粒生物探针。这种探针 同时具有小尺寸、生物功能化、稳定而高强度的荧光和良好的生物相容性这些特 点。利用这些优点，我们进而证明了这种荧光硅纳米颗粒非常适合用于癌细胞的 实时免疫荧光成像。而且，这种多肽偶联的硅纳米颗粒生物探针还可以特异性杀 伤恶性肿瘤细胞，这为同时检测和杀伤癌细胞提供了潜在的希望。 关键词：癌症检测和治疗；荧光探针；分子成像；多肽；硅纳米颗粒 作 者：宋崇喜 指导老师：何耀 教授 II II 英文摘要 多肽偶联的荧光硅纳米颗粒的体外癌细胞成像和靶向治疗研究 Peptide-Conjugated Fluorescent Silicon Nanoparticles Enabling Simultaneous Tracking and Specific Destruction of Cancer Cells in vitro Abstract Over the past decade, taking advantages of unique optical properties and favorable biocompatibility, fluorescent silicon nanoparticles (SiNPs) have emerged as a novel kind of promising fluorescent naonprobes. In comparison to relatively large-sized proteins, Arg-Gly-Asp (Arginine-Glycine-Aspartic acid, RGD) peptides, known as one typical kind of biofunctional small molecules, are thought to have great potential as targeting ligands of cancer cells due to their smaller size, higher stability, and lower immunogenicity, etc. In our research, we present a kind of fluorescent silicon nanoparticles bioprobes, i.e., peptides-conjugated SiNPs, which simultaneously feature small sizes, biological functionality, stable and strong fluorescence, as well as favourable biocompatibility. Taking advantages of these merits, we further demonstrate such resultant SiNPs bioprobes are superbly suitable for real-time immunofluorescence imaging of cancer cells. Meanwhile, malignant tumor cells could be specifically destroyed by the peptides-conjugated SiNPs, suggesting potential promise of simultaneous detection and destruction of cancer cells. III III 多肽偶联的荧光硅纳米颗粒的体外癌细胞成像和靶向治疗研究 英文摘要 Keywords: Cancer detection and treatment; Fluorescent probes; Molecular imaging; Peptides; Silicon nanoparticles Written by: Chongxi Song Supervised by: Prof. Yao H