多肽基重金属离子化学传感器的分析-分析化学专业论文.docxVIP

聊城大学硕士学位论文 使 Dansyl-P5 的荧光发生了荧光共振能量转移将 Trp 的能量转移给了 Dansyl 荧光 基团。通过不同 pH 值对它的影响研究表明，该探针在中性溶液中检测 Ag+是比 较理想的，其检出限为 0.53 μmol·L-1。因此，有望根据该多肽的这种性质来设计 能选择性结合 Ag+的荧光比率化学传感器。 关键词：重金属离子；化学探针；多肽 iii 聊城大学硕士学位论文 聊城大学硕士学位论文 PAGE PAGE iv ABSTRACT Different sequence of amino acids, the structure of peptide is changed. Structure determines its property and function of the material. To study the relationship of the structure with properties and function of peptides have important significance. It can help us to better understand the efficacy of various amino acids, so as far as possible to synthesis better physiologically active peptides. Nowadays, heavy metal ions pollution becoming a more and more serious problem. It caused serious damage for human health and ecological environment. So how to detect the heavy metal ions effectively is of great significance in environmental science, biological science and medicine, etc. Current chemical sensor used for determination of heavy metal ions development more and more quickly duo to the chemical sensor has the advantages of high sensitivity, high selectivity and real-time strong, etc. The syudy of fluorescent chemical sensors, colorimetric sensors and electrochemical sensor developed especially striking. The chemical sensor based on peptide is widely used in the field of detection of heavy metal ions. Because it prove to be a good solubility in water, can be easily sythesized via Fmoc-solid phase peptide sythesis(SPPS) and allows conjugation to appropriate systems of biological interest. Based on the principle of the chemical sensors, we have designed and synthesized five chemical sensors which based on peptide motif. Then the interaction of metal ions with them were investigated, respectivily. The main content are as follows: The investigation of a high selectivity ratiometric fluorescent chemosensor for Cd2+ A ratiometric fluorescent chemosensor Dansyl-Cys-Pro-Pro-Cys-Trp-NH2（Dansyl-P5） was synthesized by SPPS. Dansyl is fluorophore.