房颤患者血清对心肌成纤维细胞趋化运动的影响-内科学(心血管内科)专业论文.docxVIP

Effects of serum from patients of atrial fibrillation on chemotaxis of cardiac fibroblasts Master Candidate: Duan kadan Supervisor: Prof Li Yushu Department of Cardiology, Union Hospital, Tongji Medical College, Hua Zhong University of Science and Technology Abstract AIM Atrial fibrillation is a clinically common arrhythmia,which is characterized by atrial fibrosis,myocardial fibroblast is play a important role in fibrosis process.We designed this experiment to investigate the different chemotatic effects of the cardiac fibroblasts to the serum between atrial fibrillation and other control groups,and to predict the relationship between fibrosis followed atrial fibrillation and movemoment behavior of fibroblast. METHODS The selected objects was divided into four groups which were controls （SR,n=16）, non-Af atrial arrhythmia（AA,n=18）,paroxysmal Af（Paro-Af,n=19）and persistent Af（Pers-Af,n=20）,serum were detected;Cardiac fibroblasts were isolated from the ventricles of neonatal Sprague-Dawley rats (1-3days old)and primary cultured with digestion,different speed adherence methods.The cell in 3 to 4 passages were used for transwell chamber assay to determine the chemotatic effects of the cardiac fibroblasts to the serum between atrial fibrillation and other control groups,which is designed to analysis the cells through the polycarbonate membrane and atrial fibrillation in the relevance of each group,and to predict the progress of Af. RESULTS Compared with control，the cells that migrated through the polycarbonate membrane were obviously increased in Af groups.The strongest Chemotaxis was induced by the serum from the patients of persistent Atrial fibrillation (pers-Af), the numbers of migrated cells in non-Af atrial arrhythmia(AA) were higher than that in paroxysmal Atrial fibrillation（paro-Af）,that in control group was least.The multiple logistic regression analyses show that the migrated cells in the lower surface were related to atrial fibrillation and left atrial d