供者自然杀伤细胞减轻GVH并提高移植成活率的实验研究..docVIP

供者自然杀伤细胞减轻GVHD并提高移植成活率的实验研究 ：黄纯兰，刘霆，陆晓茜，孟文彤 】本研究观察激活的供、受者NK细胞能否减 轻骨髓移植后GVHD和增加移植成活率。以C57BL/6小鼠作为 供者，将接受Gy60C0Y射线全身照射的BALB/c受鼠随机分 为4组，对照1组、对照2组、实验1组和实验2组，观察 每组小鼠的活存期、嵌合率、GVHD临床和病理改变。结果 表明：对照1组全部活存，实验1组比对照2组活存期短， 实验2组比对照2组活存期延长。实验1组GVHD临床及病 理评分高于对照2组，实验2组GVHD临床及病理评分明显 低于对照2组。实验1组和实验2组嵌合率均较对照2组高， 实验2组的嵌合率比实验1组明显增加。结论：激活的供者 NK细胞能延长实验小鼠的活存期，降低GVHD严重程度，增 加移植成活率；而激活的受者NK细胞却缩短实验小鼠的活 存期，加重GVHD严重程度。受者NK细胞也增加移植成活率， 但比供者NK细胞作用小。 关键词】骨髓移植;NK细胞;GVHD;移植成活率 Experim entalStudyof DonorNatural KillerCellsR educingGraft -versus~host DiseaseandEn hancingEngra ftment Abstr actThepurpos eofthisstudy wastoinvesti gate whethe rpretranspla ntinfusionof reactivenatu ralkillercel lsfromdonoro rrecipientca nreducegraft -versus-host diseaseanden hanceengraft mentinbonema rrowtranspla ntation. Reci pientBALB/cm iceweredivid edinto4group safterrecEiv edGytotal-bo dyirradiatio n(TBI): cont rolgrouplwas treatedwithn othing,contr olgroup2recE IvedBMTalone ,experimentg rouplreceive dBMTandautor eactiveNKcel Is,experimen tgroup2recei vedBMTandall oreactiveNKc ells. Lifespa n, clinicala ndpathologic changesofGV HDand chimer ismrateofeac hgroupwereev aluated. Ther esultsshowed thatallmicew eresurvivali ncontrolgrou pi. Thelifesp anwasshorter inexperiment grouplthanth at incontrol group2andlon gerinexperim entgroup2tha nthat incont rolgroup2. GV HDwashigheri nscoreofexpe rimentgroupl thanincontro lgroup2butlo werinexperim entgroup2tha nthatincontr olgroup2. The donorchimeri sm rateinbot htwoexperime ntgroupswere higherthanth atincontrolg roup2,howeve r,thedonorch imerismrate washigherine xperimentgro up2thanthati nexperimentg roupl. Itisco ncludedthatp retransplant infusionofal loreactivedo norNKcellsca nprolonglife span, reduce thedegreeofG VHDandenhanc eengraftment . Butautoreac tiverecipien tNKcellscans hortenlifesp an, aggravat ethedegreeof GVHDandalsoe nhanceengraf tment,whichi sweakerthanthatusingallo reactivedonorNKcelIs. Ke ywordsbonema rrowtranspla ntation；natu ralkillercel l；GVHD；engra ftme