雌激素受体β介导植物雌激素骨代谢效应的流行病学与细胞学研究-药理学专业论文.docxVIP

IV IV 摘要 介导。 关键词:绝经后骨质疏松症;植物雌激素;雌激素受体；多态性；GEN; BMD;破骨细 胞；成骨细胞 Abst Abstract PAGE PAGE VI Absract Part one Relationship between estrogen receptor β gene polymorphisms and the effect of dietary phytoestrogens on bone mineral density in postmenopausal women Objective Postmenopausal osteoporosis is a comprehensive disease and characterized by low bone mineral density, and mainly base on estrogen decline. treatment of women with hormone replacement therapy can prevent bone loss. Phytoestrogens estrogenic effects and little toxicity or side effects can prevent bone loss and Maintain normal bone metabolism. Compared with western countries＇low dietary phytoestrogens consumption, the incidence of Postmenopausal osteoporosis still not decreases in our country. Thus, we suspected that genetic differences among individuals played a role. In the past our group have find that the ERα gene polymorphisms were releared with the dozes of dietary intake of phytoestrogens and Postmenopausal osteoporosis. But There is no report about the relationship between the effect of phytoestrogens anesising Postmenopausal osteoporosis and ERβ gene polymorphisms until now. So we planed to combine with estrogen receptor beta gene polymorphism and dietary intake of phytoestrogens to analyze their relationship while they effect Bone metabolism, and provide a theoretical basis for further research treatment. Methods we randomly selected 300 postmenopausal women from NanCha ng ,according to the requirement of the investigation, collected basic material a nd diatery material of the samples.BMD of different bony tissues were measur ed by dual energy X-ray absorptiometry. After calculating the ADI(acceptable d aily intake) of all kinds of phytoestrogen，we make a comparison about charac teristics and BMD according to tertiles of phytoestrogen intake. What’ more,Th e RsaI and AluI polymorphisms of ERβ gene were detected by Polymerase ch ain reaction-restriction fragment length