复方芪竭膏对大鼠创伤模型愈合影响的实验研究-中医外科学专业论文.docx

成都中医药大学 成都中医药大学 2008 级博士学位论文 PAGE PAGE 10 关键词: 复方芪竭膏 皮肤 创伤愈合 溶菌酶 细胞凋亡 Effect and mechanism of Compound QiJie Cream in rat cutaneous incised wound healing ABSTRACT objective: To observe the effect of Compound QiJie Cream ( CQJC) in rat cutaneous incised wound healing and investigate the mechanism of accelerating the rat back wound healing. Methods: two full thickness dermal excision wound models with the diameter of 1.8cm were established on the back of 72 healthy male or femal Wistar rats，which were randomly divided into 3 groups: model group ，Jing wanhong goup(Control goup) ， CQJC goup, 24 rats per group. Jingwanhong as Positive compare medicine.The rats in each group were extemal applied with corresponding drug，0.1 g each wound surface and twice a day. Tissues were removed 3,7,10and14 days after surgery.The change of histomorphology was observed by the means of hematoxylin-eosin (HE) staining, and the fibroblasts in granulation tissues of the wound in each group were observed by PCNA staining. The CD31 staining was used to study the new blood capillaries in granulation tissues. The content of EGF, TGF-β 1, Collagen-I, Fn, Collagen — III and EGFR was determined by the use of streptavidin-perosidase immunohistochemistry. The lysozyme in wound tissue was tested by the means of streptavidin-perosidase, and the fibroblast cellular apoptosis in granulation tissues was studied by TUNEL method. Images of each group were analyzed by Image-Pro-Plus image analysis software. All counted data was expressed in the form of，and processed by SPSS16.0 statistics software. Results: The wound healing rate, wound healing time, wound granulation tissue thickness, the scar area after 30days and other indicators of the CQJC were significantly heighten than Model group(P＜0.05 or P＜0.01) in each time points of observation; The new blood capillaries in the wound granulation tissues and the fibroblast cells of CQJC and Jing Wanhong group were statistically increased (P0.01)than