高产油脂菌株选育及其油脂提取工艺研究-生物化学与分子生物学专业论文.docxVIP

河南科技大学硕士学位论文 河南科技大学硕士学位论文 II II 间、提取液配比(VCHCl︰VCHOH)三个显著因子进行响应面优化，得到最佳工艺参 数为酸处理时间 34 min，超声时间 7.5 min，提取液配比(VCHCl︰VCHOH) 1.9︰1。 与原始工艺相比，湿菌油脂重量从 42.67±1.43 mg/g 提高到 57.33±2.4 mg/g，提高 了 36.5%。 关 键 词：粘红酵母，微生物油脂，诱变选育，工艺优化 论文类型：应用基础研究 摘 要 Subject: Study on Oil High-yield Strains and Tecnichs of Extracting Microbial Oil Specialty: Biochemistry and Molecular Biology Name: Wang Min Supervisor: Li shichang ABSTRACT Oil which has a wide range of applications is one of primary substance that can make up and sustain life activity, and is important industrial raw materials. Currently, oil source is mainly from animals and plants.As microbial oil was studied by people, utilization of microbial fermentation was used to obtain oils, which not only can alleviate the situation of scarce resources of animal and plant oils, but also can reuse waste resource by oil-producing strains.That provides a new way for access to oil resources. Taking Rhodotorula glutinis 31596 as the research object in this paper, high- yielding strains of oil were obtained by three means, such as medium optimization, bacterial mutation and optimization of oil extraction. Major researches were as follows: Optimization of oil producing medium By studying, best fermentation medium components of Rhodotorula glutinis 31596 were as follows: glucose 80 g/L, (NH4)2SO4 1.11 g/L, KH2PO4 2.5 g/L, peptone 1.8 g/L, Under culture conditions: temperature 28 ℃, initial pH 5.8, inoculum size 5%, 250mL flask containing 40 mL medium. Its biomass and oil production respectively reached 18.18±3.34 g/L and 32.06±1.91 mg/g. Mutation breeding of high-yield strains of oils In order to further improve bacterial biomass and oil production of Rhodotorula glutinis 31596, low-energy ion implantation mutation and UV mutation were used to screen high-yield strains. Finally, one mutant strain VB19 was obtained. Biomass and oil production of this strai