无花果内生真菌分离鉴定和其活性研究.docVIP

无花果内生真菌分离鉴定及其活性研究 马养民1?基金项目：国家自然科学基金(No和教育部科学技术研究重点项目（ ?基金项目：国家自然科学基金(No和教育部科学技术研究重点项目（No.208144）资助. ?马养民 （1963—），男，博士，教授，博士生导师，长期从事天然产物化学研究工作.Tel: (029) E-mail: HYPERLINK mailto:mym63@ mym63@ （1. 陕西科技大学教育部轻化工助剂化学与技术重点实验室，西安 710021; 2. 山西大同大学农学与生命科学学院，山西大同 037009） 摘 要：[目的]分离和鉴定无花果(Ficus carica)植物内生真菌，并比对其进行活性筛选。[方法]采用形态学方法对分离菌株进行鉴定，以金黄色葡萄球菌、大肠杆菌、白菜黑斑病菌、苹果腐烂病菌、小麦赤霉病菌、草莓枯萎病菌、青霉菌为测试菌对分离得到的植物内生真菌进行抗菌活性实验。[结果]从健康的无花果植物的根、茎、叶中分离共获得61株内生真菌。属于5纲，7目，12科，20属。抗菌活性试验表明有40株内生真菌具有抗菌活性,占总内生真菌的65.57%。 [结论]无花果植物内生真菌多样性丰富，具有很好的研究和应用价值。 关键词：无花果；内生真菌；分离；鉴定；抗菌活性 Isolation and Identification of Endophytic Fungi from Ficus carica and Their Antimicrobial Activity MA Yang-min, XU Xiao-na, ZHANG Hong-chi （1. Key Laboratory of Auxiliary Chemistry Technology for Chemical Industry, Ministry of Education. Shaanxi University of Science Technology, Xian, 710021, China； 2. College of Agronomy and Life Science, Shanxi Datong University, Datong, Shanxi 037009, China) Abstract: [Objective] The aim of this study was to isolate the endophyte of Ficus carica and screen of its antibiotic activities. [Method] The endophytic fungi were indentified by morphology, Staphylococcus aureus, Escherichia coli, Alternaria brassicae, Valsa mali, Fusarium Graminearum, Fusarium oxysporumf. sp .fragariae, Penicillium notatum were selected as the indicator to test the inhibitory activity of the isolated strains.[Result] Sixty-one strains of endophytic fungi were isolated from the healthy root, stem and leaf of Ficus carice, which belonged to twenty genera, twelve families, seven orders and five classes. Of them forty strains were antimicrobial, The percentage of antimicrobial strains to tested strains is 65.57%. [Conclusion] It indicated that the endophyte of Ficus carica were diversity and rich and has the best research and application value. Key words: Ficus carica; Endophytic fung; Isolation; Identification; Antimicrobial activity 无花果(Ficus carica)系桑科无花果属的多年生木本植物[1]，是中国传统医学中一种重要的