蛋白质相互作用预测方法的研究与蛋白质超二级结构预测系统的开发生物信息学专业论文.docxVIP

AbstractABSTRACT Abstract ABSTRACT Identification of protein-protein interactions and protein structures is crucial for elucidating protein functions and further understanding various biological processes in a eeU．It has been the focus of the post-proteornic researches．With a huge amount of protein sequences information provided by genome—sequencing projects，there is a growing demand for developing advanced computational methods for predicting protein-protein interactions and protein structures by using sequence information only． In this paper,we developed computational methods for protein-protein interactions prediction and protein supersecondary structure prediction．This thesis mainly includes the following facets： (1)We propose a sequence-based method based on a novel representation of local protein sequence descriptors for protein-protein interactions prediction．Local descriptors account for the interactions between residues in both continuous and discontinuous regions of a protein sequence，therefore this method is capable of capturing multiple overlapping continuous and discontinuous binding patterns within a protein sequence．When combined witll the k-nearest neighbors learning system and performed on the PPI data of S．cerevisiae，the proposed method achieved excellent results．Meanwhile，the final prediction model Was tested using the independent data set of the E．coli PPIs with a good performance．The performance of the combination between this novel representation of local protein sequence descriptors and SVM was also evaluated using the independent dataset from E．coli and the results indicate the stable performance of the proposed representation of local protein sequence descriptors when combining with different machine learning techniques．Given the complex nature of PPIs，the performance of our method is promising and it Call be a helpful supplementary tool for PPIs prediction． (2)We present a new method to predict 13-hairpins in proteins by combining the radia