乙醇-硫酸铵双水相体系萃取坛紫菜多糖-食品科学.PDFVIP

46 2014, Vol.35, No.22 食品科学 ※工艺技术 乙醇-硫酸铵双水相体系萃取坛紫菜多糖 张鉥孟，陈美珍* （汕头大学理学院生物系，广东汕头 515063 ） 摘  要：探究不同系线长度的乙醇-硫酸铵双水相体系对坛紫菜多糖萃取的影响。采用浊点滴定法绘制乙醇-硫酸铵 双水相相图，选取不同系线长度体系，测定其对紫菜多糖分配情况。结果表明：在乙醇质量分数27%、硫酸铵质量 % R % K 分数18 （即系线长度为40 ）、体系相比 为1.1时，紫菜多糖的回收率可达79.8 ，分配系数 为0.23 ；其蛋白去除 率明显高于传统醇沉法（双水相萃取多糖蛋白含量为1.20%，醇沉多糖蛋白含量为6.17% ），且具有较好的脱色效 果，可用于紫菜多糖的分离纯化。 关键词：双水相；系线长度；坛紫菜；多糖 Extraction of Polysaccharides from Porphyra haitanensis Using Ethanol-Ammonium Sulfate Aqueous Two-Phase System * ZHANG Shu-meng, CHEN Mei-zhen (Department of Biology, College of Science, Shantou University, Shantou 515063, China) Abstract: The effect of tie line length (TLL) of ethanol-(NH ) SO aqueous two phase system (ATPS) on the extraction 4 2 4 of polysaccharides from Porphyra haitanensis was investigated. The phase diagram was obtained by cloud point titration, and then different TLLs of ATPS were selected and partition coefficients of polysaccharides were detected. The yield and partition coefficient of polysaccharides were 79.8% and 0.23, respectively, using a system consisting of 27% ethanol and 18% (NH ) SO (forming a TLL of 40) with a top/bottom phase volume ratio of 1.1. The removal rate of protein was 4 2 4 significantly higher than that reported with traditional ethanol precipitation method (1.20% vs 6.17%). Meanwhile, the proposed method had good decolorization effect. Thus, it can be used for the separation and purification of polysaccharides from Porphyra haitanensis. Key words: