甘蓝型油菜BnFADC5基因启动子及内含子在表达水平的-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2016, 42(10): 14711478 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2016.01471 甘蓝型油菜BnFAD2-C5 基因启动子及内含子在表达水平的功能分析 刘睿洋 刘 芳 张振乾 官春云* / , 410128 : , (FAD2 )BnFAD2-C5 , , 1257 bp , GUS GFP , GUS –319 ~ –1 bp ; Western blot , BnFAD2-C5 –1257 ~ –1020 bp –319 ~ –1 bp , BnFAD2-C5 , +631~ +1033 bp : ; BnFAD2-C5 ; ; Functional Analysis of BnFAD2-C5 Promoter and Intron at Expression Level in Brassica napus    LIU Rui-Yang , LIU Fang , ZHANG Zhen-Qian, and GUAN Chun-Yun College of Agriculture, Hunan Agricultural University / National Oilseed Crops Improvement Center in Hunan, Changsha 410128, China Abstract: High oleic rapeseed breeding and the formation mechanism of oleic acid have become a central issue after finding the important economic value of rapeseed oil with high oleic acid. The fatty acid dehydrogenase gene (FAD2) is a key enzyme gene to control oleic acid content, but the regulation of FAD2 gene is not well understood. According to the homology between rapeseed and oleracea, the BnFAD2-C5 promoter sequence of 1257 bp was cloned. Promoter and intron of BnFAD2-C5 gene were analyzed using β-glucuronidase (GUS) reporter and green fluorescent protein (GFP) reporter system to construct deleted vectors and trans- form Arabidopsis thaliana. Deletion analysis of BnFAD2-C5 promoter through GUS stainning revealed that –319 to –1 bp was the minimum promoter region. And deletion analysis of BnFAD2-C5 promoter and intron through GFP reporter system using western technique showed that –1257 to –1020 bp and –319 to