减少大鼠肾缺血再灌注损伤的方法对比分析-麻醉学专业论文.docxVIP

IV IV 摘要 组与 Sham 组比较，肾组织中 SOD 活性明显降低（p0.05）；IPostC 组、RIPC 组、肌苷组和 HC-A 组肾组织中 SOD 活性与 IR 组对比则增高了（p0.05）；再 灌注 1 周后，HC-A 组与 Sham 组比较，SOD 活性降低（p0.05）。 结论： IRI 肾病理表现为肾组织细胞结构和功能异常，Cr 和 BUN 升高，MDA、 MPO 活性升高，SOD 活性下降；实验结果显示，IPostC、HC-A 肾保护液、RIPC 及肌苷注射液处理等对 IRI 肾具有保护作用；其可通过降低 MDA 和 MPO 酶活 性，提高 SOD 酶清除氧自由基能力等抗氧化机制，而达到保护肾功能作用。研 究表明 RIPC 对 IRI 肾有保护作用，且和 IPostC、肌苷注射液处理比较无明显差 异，较 HC-A 肾保护液处理对 IRI 肾更具有保护作用，因其操作简易、无创伤、 效果明确等优点，因此具有更广阔的临床应用前景。 关键词：缺血后处理；远程缺血预处理；肌苷注射液；肾保护液；缺血-再灌注 损伤；抗氧化。 Abstract Abstract PAGE PAGE VI ABSTRACT Objectives: In this study, clamping the renal artery of the SD rats turns to renal ischemia-reperfusion injury (ischemia reperfusion injury, IRI) model, useing remote ischemic and preconditioning ischemia treatment (ischemic postconditioning, IPostC)、methods of inosine injection and HC-A renal protective perfusion treatment before and after reperfusion (remote ischemic preconditioning, RIPC), 24 hours, 48 hours, and 1 week after ischemia-reperfusion we detected blood urea nitrogen (BUN) and serum creatinine (Cr) in rat inferior vena. With histopathological wo can evaluate the degree of injury, and detective renal tissue myeloperoxidase (Myeloperoxidase, MPO), superoxide dismutase (Superoxide dismutase, SOD) activity and malondialdehyde (Malondialdehyde, MDA) activity to assess the extent of tissue peroxidation damage, comprehensive comparison of the protective effect of these types of treatment of renal IRI. Methods: Selection of adult female SD rats weighing approximately 220 ± 30g, were randomly divided into sham operation group (Sham group), IR group, RIPC group, IPostC group, inosine injection treatment group (Inosine group) and HC-A kidney protective solution perfusion group (HC-A group). SD rats in each group open right kidney removed, occlusion of the left renal artery 45 minutes after the resumption of perfusion, and kidneys of renal artery pulsatility color changes, renal artery recovery pulse, kidney change color from dark red, indicatin