姜黄素结合递增负荷有氧运动对中老年大鼠骨骼肌线粒体功能的影响-运动人体科学专业论文.docxVIP

ABSTRACTIncreasing ABSTRACT Increasing load aerobic training rats model，this paper extracts the mitochondria inside the rat quadriceps， determination and energy metabolism related biochemical indicators， representatiVe for the application of curcumin anti—aging provide some experimental basis． Research purposes： based on the determination of curcumin combined with incremental load after aerobic exercise on aging rat skeletal muscle mitochondria inhibits PTP opening degree，mitochondrial respiratory chain complex enzyme complex III， IV activity and the change of ATP enzyme activity， and were observed under electron microscope mitochondria structure changes， analyze each aging rats．Curcumin combined with incremental load aerobics on aging effect of mi tochondrial funct ion in rats． Methods：21 months only 40 male rats，adaptability to rest 3 days，adapt ive training for five days SD male rats were randomly divided into 4 groups，respectively is：quiet control group(N)，the curcumin group group(I) (C)， incremental load increasing， curcumin +load aerobic exercise group(A)，each group of 10，only 2 per component cage，each cage 5 only．Groups(I)and increasing load of curcumin+aerobic exercise group(A)increasing load using m 万方数据 the the treadmi ll exercise，increasing load experimental animal model(grade 0．，increasing exercise intensity to 21 m／min X 45 min，exercise intensity is equivalent to 75％V02max)， continuous training for five days A week，A total of 8 weeks．Groups of rats in 24 h after the last training session anesthesia，broken neck death，materials．Wi l l be fixed on the operating table of rats after death，cut the skin of lower l imb，careful handl ing mouse hair，avoid sticking oh the muscle，quickly remove the quadriceps．In the cold saline to remove fat and other connective tissue， filter paper blot，put in liquid nitrogen freezing，一80℃ low temperature preservation．Using colorimetric method were determined mitochondrial atpase activity and mitochondrial respirat