胶原酶敏感性聚α羟基酸基仿生材料合成-化学专业论文.docxVIP

重庆大学硕士学位论文中文摘要 重庆大学硕士学位论文 中文摘要 I I 摘 要 聚乳酸具有良好的生物相容性、生物降解性、力学性、材料加工性及可消毒 性，广泛应用于生物医学工程领域。为了调控聚乳酸类材料的降解速度以便与工 程化组织的形成速度相匹配，众多学者研究了聚乳酸共聚、共混、复合等改性， 但是，这些方法没有改变聚乳酸类材料的降解特征与机制。欲匹配工程化组织形 成与材料降解速度，仿生应该是最有效的方案。 本论文以自制 D,L-丙交酯为原料，以赖氨酸和胶原酶敏感肽作引发剂，丙交 酯开环聚合制备双端羟基聚乳酸寡聚酯，实现胶原酶敏感性聚乳酸基仿生材料的 合成。利用紫外-可见分光光度计、傅里叶变换红外光谱仪（FTIR）、氢核磁共振 波谱仪（1H-NMR）和化学分析等方法对聚乳酸寡聚酯进行分析了表征。主要研究 内容和结论如下： (1)以 D,L-丙交酯为原料，辛酸亚锡作为催化剂，赖氨酸可以引发 D,L-丙交酯 实现开环聚合，且聚合物为双端羟基聚乳酸寡聚酯（HO-PLA-Lys-PLA-OH）。 (2)应用正交试验优化了赖氨酸引发丙交酯开环聚合条件，当聚合温度为 110℃、反应时间 60h、辛酸亚锡的质量分数为丙交酯的 1.25%、丙交酯与赖氨酸 的摩尔比为 41 时，双端羟基聚乳酸寡聚酯的分子量（5618g·mol-1）与设定的理论 分子量最接近。 (3)根据多肽（GGGLGPAGGK）与赖氨酸均含有双端-NH2 的特点，采用赖氨 酸引发丙交酯开环聚合条件，以多肽引发丙交酯聚合，能够获得预期的胶原酶敏 感性多肽-聚乳酸寡聚酯（HO-PLA-GGGLGPAGGK-PLA-OH）。为进一步扩链制备 高分子量的胶原酶敏感性聚乳酸基仿生材料提供了原料保障。 关键词：聚乳酸，胶原酶敏感性多肽，开环聚合，仿生，组织工程 重庆大学硕士学位论文英文摘要 重庆大学硕士学位论文 英文摘要 II II ABSTRACT Poly(lactic acid) or polylactides (PLA) was widely used in biomedical engineering due to its biocompatibility, biodegradability, as well as mechanical, machining and antiseptic properties. In order to match the degradation rate of polylactides and formation rate of engineered tissue, a great number of experiments based on copolymerization, blending and composite were studied. However, the characteristics and mechanism of degradation of polylactides did not change anymore. To match the rate of material degradation and engineerd tissue formation, biomimetic should be the most effective solution. In this thesis, oligomers of dihydroxyl-terminated polylactides was prepared by ring-opening polymerization of D,L-lactide(LA) with lysine and collagenase-sensitive peptide as initiators. The obtained oligomers were characterized with UV-vis spectrophotometer, Fourier infrared transform spectrometry(FTIR), Hydrogen nuclear magnetic resonance (1H-NMR), and chemical analysis methods. The main works and conclusions were summarized as follows： Firstly, polylactides oligomers can be obtained by ring-opening polymerization of D,L-lactide with stannous octoate as catalyst and lysine as initia