离子束重组菌DOB073的比较基因组学研究.docxVIP

离子束重组菌D0B073的比较基因组学 研究 范伟伟毛培宏蔡长龙 新疆大学物理科学与技术学院离子束生物技术中心 西安工业大学离子束生物工程与生物多样性研究中 摘要： 为了深入认识低能离子注入驱动DOB基因组的突变机制，我们利用生物信息学 方法，对离子束重组菌株D0B073进行了比较基因组学研究。结果表明，该菌株 的基因组比原始菌株增加了 12 883 bp,基因数目也比原始菌株增加了 28个，但 也缺失了原始菌株基因组中的13个基因。差界基因分析及其功能注释表明，离 子束重组菌株D0B073基因组中新基因的功能主要与能量代谢、环境适应和DNA 损伤修复相关，新基因主要分布于基因组的前部和后部;离子束重组菌株 D0B073基因组屮缺失的基因的功能主要与染色体分离蛋白和氯化物通道家族蛋 口相关，主要分布于基因组的中部。基因组系统发育与进化的分析表明，离子束 重组菌株D0B073与原始菌株亲缘关系最近。差界基因的COG功能富集分析表明, 离子束重组菌D0B073独有的基因功能明显富集于蛋白质翻译、核糖体结构和发 育、细胞壁/细胞膜/胞外层发育、防护机制，而碳水化合物转运与代谢则明显存 在于原始菌株的独有基因屮。本研究为低能离子注入驱动微生物的全基因组突变 与重组提供了一定的分子证据，也为低能离子注入驱动新基因的从头起源研究 提供了一定的理论和实践依据。 关键词： 离子束重组DOB;基因组;新基因;注释;功能; 作者简介：毛培宏，phmao@china. com; 作者简介：蔡长龙，changlongcai@126. com 基金：国家自然科学基金(No.No. Comparative Genomic Research of Recombinant Bacteria D0B073 Obtained by Ion Beam Fan Weiwei Mao Peihong Cai Changlong Research Center of Ion Beam Biotechnology， College of Physics Science and Technology, Xinjiang University; Research Center of Ion Beam Biotechnology and Biodiversity, Xi an Technological University; Abstract： In order to deeply understand the mutat ion mochanism of DOB genome driven by low-energy ion beam implantation, we carried out the comparativc genomics study of the ion beam recombinant bacteria D0B073 by using bioinformatics methods. The results showed that the genome of D0B073 was 12 883 bp higher than that of the original strain, and the number of genes was 28 more than the original strain, but it also lacked 13 genes which was exis ted in the original st rain genome- The differential gene analysis and functional annotation suggested that the functions of new genes in recombinant bacterial strain D0B073 were mainly related to energy metabolism, environmenLal adaptation and DNA repair, and these novel genes were principally distributed in the front and back of D0B073 genome;the functions of delctcd genes in recombinant bacterial strain D0B073 were mostly relevant to chromosome segregation protein and chloride channel family protein, and these genes