磁共振血管造影在磷酸二酯酶4D基因多态性与缺血性脑卒中相关性研究中的应用.docxVIP

磁共振血管造影在磷酸二酯酶4D基因多态性与缺血性脑卒中相关性研究中的应用 ［摘要］日的 探讨磷酸二酯酶4D (PDE4D)基因多态性与缺血性脑卒 中(IS)患者磁共振血管造影(MRA)结果的相关性。方法 应用聚合酶 链反应-限制性片段长度多态性(PCR-RFLP)方法检测245例IS患者和208 例正常対照组PDE 4D基因SNP83及SNP87酶切多态性。同时开展MRA检 查结果与PDE 4D基因SNP83及SNP87多态性相关性分析。结果245例 IS患者中，77. 78%患者MRA结果异常，其中，颅外血管(ECMRA)异常占 53. 37%,颅内(ICMRA)异常占65. 80%,两者兼有占41.97%。脑卒中患者 的PDE 4D基因SNP 83的CC基因型频率较正常对照组高(OR为3. 57,95%CI 1. 39-6. 52, P 0. 05)o结论PDE 4D基因SNP 87多态性与MRA结果异 常相关。PDE 4D基因SNP 83与IS相关，与MRA结果异常无显著性相关。 ［关键词］磷酸二酯酶4D基因多态性；缺血性脑卒中；磁共振血管造 影 ［中图分类号］R743. 1 ［文献标识码］A ［文章编号］1673-7210 (2014) 11 (a) -0018-05 Phosphodiesterase 4D gene polymorphism correlation with MRA findings in patients with ischemic stroke WANG Ruixia YANG Jia ZHANG Zhelin Department of Neumlogy, the Affiliated Hospital of Inner Mongolia Medical Universily, Inner Mongolia Autonomous Region， Huhhot 010050, China [Abstract] Objective To investigate the correlation between phosphodiesterase 4D (PDE 4D) gene polymorphism and MRA findings in patients with ischemic stroke (IS)? Methods Three polymorphisms SNP 83 and SNP 87 in PDE 4D gene were analyzed in 245 patients of IS and 208 controls using polymerase chain reaction—restriction fragment length polymorphism method in a case-control study. Meantime to carry out the polymorphisms SNP 83 and SNP 87 in PDE 4D gene correlation with MRA findings in patients with ischemic stroke. Results Among the 245 patients, MRA was abnormal in 77. 78% patients； extracranial MRA (ECMRA) in 55. 37%, intracranial MRA (ICMRA) in 65. 80% and both were abnormal in 41. 97% patients. The frequency of CC genotype of PDE4D83 was significantly higher in the patients with ischemic stroke compared to controls (OR = 3. 57, 95%CI 1.39-6.52, P 0.05)? Conclusion The PDE 4D gene SNP 87 polymorphism is associated with MRA abnormalities, but SNP 83 is associated with IS and not significantly related to MRA abnormal ities. [Key words] Phosphodiesterase 4D gene polymorphism； Ischemic stroke； Magnetic resonance angiography 缺血性脑卒中(ischemic stroke, IS)是由多种遗传与