EB病毒BARF1基因表达对胃癌细胞株SGC7910生物学行为.doc

PAGE PAGE 1 EB病毒BARF1基因表达对胃癌细胞株SGC7910生物学行为 【摘要】目的：了解EB病毒BARF1基因表达对胃癌细胞生物学行为的影响。方法：构建pSG5/BARF1真核表达载体，转染到EB病毒阴性的胃癌细胞系SGC7901，经RT睵CR鉴定，获得BARF1稳定表达的细胞克隆。以pSG5空载体转染细胞为对照，进行细胞凋亡诱导实验、细胞增殖实验、软琼脂集落形成实验和细胞迁移实验。结果：与对照组相比，BARF1表达细胞组经抗癌药顺铂诱导后的细胞凋亡率显著降低（Plt;0.01），增殖速度显著加快（Plt;0.01），软琼脂集落形成率显著提高（Plt;0.05），细胞迁移能力显著增强（Plt;0.05）。结论：BARF1基因能够全面增强胃癌细胞的肿瘤原性，可能在EB病毒相关胃癌发生与演化的整个过程中起重要作用。 【关键词】EB病毒；BARF1；胃癌细胞；细胞生物学行为 EffectofEpstein睟arrvirus瞖ncodedBARF1geneexpressiononthebiologicalbehaviorsofgastriccancerlineSGC7901 LIYou瞦iong1,ZHANGXue瞴i2,ZENGJian1,CHENQiao瞝in3, LILiang瞛u4,CHENGJing4,ZIRui瞗eng5,ZHOUTian瞛i1 (1.DepartmentofPathogenicBiology;2.DepartmentofBiochemistry;3.InstituteofMedicalLaboratoryScience;4.CentralLaboratory;5.UndergraduateinSpecialtyofMedicalLaboratoryScience,Grade2005,Class1,SchoolofMedicalScienceandLaboratoryMedicine,JiangsuUniversity,ZhenjiangJiangsu212013,China) ［Abstract］Objective:ToinvestigatetheoncogenicfunctionsofEBV瞖ncodedBARF1geneingastricepithelialcells.Methods：ApSG5/BARF1expressionvectorwasconstructedandtransfectedintoanEBVnegativeandmoderately瞕ifferentiatedgastriccancercelllineSGC7901.ThestablytransfectedcellclonesweregeneratedbylimitingdilutionandG418screening,andBARF1expressionwasconfirmedbyRT睵CR.ThebiologicalbehavioranalysiswereconductedincludingcellsurvivecurvedrawingandapoptosisobservationafterHoechst33342staininginducedbyanti瞔ancerdrugdiamminedichloroplatinum(DDP),cellgrowthcurvedrawing,soft瞐garcolonyformationassay,aswellascellmigrationtestunderthecontrolofvacantpSG5vectortransfectedclones.Results：BARF1expressioningastriccancercellsledtomoreresistanttoapoptosisinducedbyDDP,fasterproliferation,highercolonyformationrateinsoftagar,andstrongermigrationcapability.Conclusion：BARF1genehadallaroundoncogenicabilityingastricepithelialcellsandmayplayanimportantroleinthewholeprocessofoccurringandpromotingEBV瞐ssociatedgastriccarcinoma. ［Keywords］Epstein睟arrvirus;BARF1;gastriccancer;cellbiologicalbehaviorEB病毒（Epstein睟arrvirus,EBV）属于人γ疱疹病毒，与多种恶性肿瘤相关，如Burkitt淋巴瘤