光交联羟丙基壳聚糖水凝胶材料的制备及其应用-药剂学专业论文.docxVIP

II II 敷料，具有合适的黏附性、伤口顺应性、阻菌性及透气性；下层为海绵状光交联 羟丙基壳聚糖支架，能够促进成纤维细胞的粘附及其生长。因此这种双层结构的 壳聚糖生物材料能够作为治疗全程皮肤损伤的伤口敷料或皮肤替代物。 关键词：壳聚糖，水凝胶膜，支架，伤口敷料，组织工程 III III Abstract A photocrosslinkable azide hydroxypropyl chitosan(Az-HPCS) was prepared by introducing photoreactive azide groups (4-azidobenzoic acid, Az-) to the amino groups of hydroxypropyl Chitosan (HPCS), a water-soluble chitosan derivate, Its structure and Az- substitution degree was characterized by NMR, FT-IR, elemental analysis. Az-HPCS solution (25 mg/ml) was crosslinked to form hydrogel under UV irridiation (4w, 254nm, 90sec). We are interested in the potential of this hydrogel as wound dressing, tissue engineering scaffold, and multi-layer skin substitute. We characterized the in situ formed hydrogel membrane for evaluating its potentiality as a wound dressing. Generally, in situ formed hydrogel dressings create minimally invasive methods that offer advantages over the use of preformed dressings such as conformability in any wound bed, convenience of application, and improved patient compliance and comfort. The hydrogel membrane is stable, flexible, and transparent, with a bulk network structure of smoothness, integrity, and density. Fluid uptake ability, water vapor transmission rate, water retention and adherence strength of the resulted hydrogel membranes (0.1mm thick) were determined to range from 97.0~96.3%, 2934~2561g/m2/day, 36.69~22.94% (after 6 days), and 4.8~12.3N/cm2, respectively. These data indicates that the hydrogel membrane can maintain a long period of a moist environment over wound bed for enhancing re-epithelization. Specifically, these properties of the hydrogel membrane were controllable to some extent, by adjusting the substitution degree of the photoreactive azide groups. The hydrogel membrane also exhibited barrier function, as it was impermeable to bacteria but permeable to oxygen. In vitro experiments using two major skin cell types (dermal fibroblast and epidermal keratinocyte) revealed the h