基质金属蛋白酶-9单核苷酸多态性与冠状动脉粥样硬化的关联研究-内科学专业论文.docxVIP

摘要目的：本课题旨在从分子遗传水平探讨基质金属蛋白酶一9 摘要 目的：本课题旨在从分子遗传水平探讨基质金属蛋白酶一9 (Matrix Metalloproteinases一9，MMP一9)rs3918242基因多态性与 冠状动脉粥样硬化的关系。 对象与方法：连续选择2003年9月22日至2003年11月28日 在中南大学湘雅医院心导管室行冠状动脉造影的患者111例，其中， 冠状动脉造影(CAG)阳性患者68例，男50例，女18例，平均年龄 62．17±8．00岁(44—78岁)；冠状动脉造影阴性患者43例，男25例， 女18例，平均年龄59．02±9．87岁(35—76岁)。DNA采用酚／氯仿法 从外周血白细胞中提取。MMP一9rs3918242基因多态性则经聚合酶链 反应(polymerase chian reaction，PCR)及PaeI限制性核酸内切酶 消化检测。 结果：CAG阳性组中MMP-9rs3918242基因c／T及T／T基因型频 率分布明显高于CAG阴性组(pO．05)。不同程度冠状动脉病变组中 姗P-9rs3918242各基因型频率分布无明显差异(pO．05)。不同支 数冠状动脉病变组中MMP-9rs3918242各基因型频率分布无明显差异 (p0．05) 结论：本研究结果提示冠状动脉粥样硬化的发生与基质金属蛋白 酶-9(MMP-9)rs3918242基因多态性有相关性，MMP-9rs3918242基 因T等位基因可能是冠状动脉粥样硬化的一项独立易患因素； MMP一9rs3918242基因多态性与冠状动脉狭窄程度及病变支数无相关 性，对冠状动脉病变程度无预测价值。 关键词基质金属蛋白酶，基因多态性，冠心病，动脉粥样硬化 ABSTRACTOBJECT ABSTRACT OBJECT l VE：to investigate the association of Matrix Metalloproteinases-9(1VIlVlP-9)rs39 1 8242 gene polymorphism with coronary atherosclerosis． ME I HODS：We studied a cohort of 1 1 1 consecutive subjects undergoing coronary angiography(CAG)in Xiang ya hospital from 9／22／2003 to 1 1／28／2003．Of the 1 1 1 patients studied，68(the case group，male 50，female 1 8，aged 44—78)had angiographically documented coronary stenosis in at least one major epicardial coronary artery，and 43 (the control group，male 25，female 18，aged 35-76)were angiographically normal．DNA was extracted from white cells using the phenol／ chloroform method．the Matrix MetaUoproteinases-9 (MMP一9) rs391 8242 gene polymorphisms were analyzed using polymerase chain reaction-sequence specific primers(PCR-SSP)technique with paeI (endonuclease)enzyme cutting methods． RESULTS：There was a significant difference in MMP-9 rs391 8242 CT polymerphism between the coronary stenosis group and non-stenosis group，such that the frequencies of the C／T and T／T genotypes of the lVIMP一9 rs3918242 CT polymorphism were significantly higher in the former than the latter groups(PO．05)．For the patients with different severity of coronary artery lesions，there Was no sig