基质金属蛋白酶-9在子宫内膜异位症血清和腹腔液中的测定及临床意义-妇产科学专业论文.docxVIP

中文摘要基蒺金麓蛋白酶*露在子宫肉膜舅德症盘清鞠腥霾液中麴 中文摘要 基蒺金麓蛋白酶*露在子宫肉膜舅德症盘清鞠腥霾液中麴 测定及l隘床意义 妇产辩颂士硬究生 剃素琴 瞥师 戴淑真教授 中文摘要 爨麓 狳讨蠢潘及羧靛渡中蒺凄会藕蛋鑫鹣～9(MMP一9)懿含量及 其意义。 方法 应箱酶联免疫吸辩试验(enzyme—linked immunsorbent assay， ELISA)测定25例EMS病人(其中I期一II期8例，IIl期一I＼『期1 7 秘)及16藕正常对照精血清及黢承中MMP．9含嚣。 结果(1)EMs燃及对照缝斑潦MMP．9的中位数分裂必134+10ng／ml、 68．30ng／ml，范围分别为105．50ng／ml～253．60ng／ml、50．40ng／ml～ 80．20ng／ml。EMs及对照缀艨东MMP一9中位数分雕炎8，50ng／ml、 3．25ng／ml，范潮分剐为7．20ng／ml～8．75ng／mt、2．51ng／ml～3．50ng／ml。 (2)EMs组血清及黢水MMP一9驰水平分剐聪显赢于对照缀，差异农 裰显著髋(P0．01)。(3)EMs组血清与疆承之闯MMP一9表达承平有撼 落楗关性，(Spearman等级提关PO。01)。(4)对照组敷溃与腹水之阕 MMP．9表达水平有明显的稠关往，(Spearman等级榴关P《o．01)。 (5)EMS组MMP一9水平与临床分期积月经周期谢关。衄瀵MMP一9的 承平在粼耢、Ⅳ麓高予i蒴、i{期，分泌翳离于增生麓，差羿均有显著 憾(PO。05)；腹水中MMP-9的水平在月经周期中分泌期表达越于增生 辩，差孬有盈藩性户0．05)，R-AFS分期阚差弄茏显著豫(P O．05)。(7)EMs组血渍及腹腔液MMP一9水平与临床分期(R-AFS)及痛 经评分秃显著鞠关槎(尹0．05)。 结论 EMs患鬻盘瀵中MMP．9东平与予露蠹膜弊使症发生发疑有关， 腻水中MMP．9水平与闫经周期有关，但与临床分期无关。 关键逮 基臻金矮蛋塞酶；MMP一9；子赛肉膜异健疰；毯LlSA 英文摘要Expression 英文摘要 Expression and clinical value of matrix 黼e重alloprolei珏ase一9 in serum and ascites in endometriosis Abstract Objective：To detect the levels of preoperative serum and operative ascites matrix metailoproteinase一9 in patients with endometriosis，and to investigate which play the role in diagnosis and pathogenesis of endometriosiS． Methods： MMP一9 contents in peritoneal ftuid (PF)and serum of 25cases with EM were detected by enzyme*linked immunosorbent assay (ELISA) and compared with the counterparts of l 6 cases without EM (controls)．The preoperative serum and ascites obtained at the time of surgery or laparoscopic． Results： (1)The median and ranges of serum MMP-9 levels in EMs group and control group were as follows：EMs group：134+10ng／ml、 l 05．50ng／ml～253．60ng／ml；control group：68．30ng／ml、50．40ng／ml～ 80．20ng／m1．The median and ranges of ascites MMP-9 levels in EMs group and control group were as follows：EMs group：8．50ng／ml、7．20ng／ml～ 8 75ng／ml；control group：3．25ng／ml、2．51ng／ml 3。50ng／ml。2)There are obvious significant diffence between EMs and control(P《O．01)。(3)The serum a