活血化瘀、益气活血方药对实验性玻璃体积血抗氧化作用的探讨-中医五官科学专业论文.docxVIP

Y Y 1007834 中文摘要 目的：通过建立实验性玻璃体积血动物模型，比较活血化瘀、益气活血方 药对家兔实验性玻璃体积血抗氧化损伤的作用探讨。 方法：采用“兔耳缘静脉取血，直接玻璃体腔注射”的方法建立玻璃体积 血模型。通过玻璃体内SOD、MDA含量测定，观察活血化瘀、益气活血两种不同 治法的方药在对玻璃体积血抗氧化损伤作用的异同。 结果：造模后即见玻璃体内红色血性混浊，眼底不能窥见，3天后出现片状 混浊，眼底窥不见，14至30天部分兔眼可见玻璃体腔絮状混浊，眼底模糊， 周边可窥及部分眼底；模型对照组玻璃体MDA含量明显高于正常对照组(pO．01)。 SOD活性明显低于正常对照组(p0．01)；活血化瘀和益气活血在提高SOD活性、 降低MDA含量明显优于模型组(p0．01)；两组间比较无统计学意义(p0．05)。 结论：两组药物均能通过促进玻璃体积血的吸收，减轻玻璃体的混浊，减 轻视网膜及玻璃体组织的氧化损伤，但两用药组之间比较无统计学意义。 关键词：玻璃体积血 动物模型 活血化瘀 益气活血 超氧化物歧化酶(SOD) 丙二醛(MDA) ABSTRACTobjective：Through ABSTRACT objective：Through establishing the experimental animal model of vitreous hemorrhage，compare the different mechanism of preventing and curing the Rabbits eyes vitreous hemorrhage and the damnification to retina and vitreous between using the presc却tions which is for removing blood stasis by promoting blood circulation and for supplementing Qi to promoting blood circulation． Methods：Establish the vitreous hemorrhage animal model by injecting the blood from也e rabbits ear-rim vein into its vitreous．Measure the concentration of the vitreous’SOD and MDA．Compare the different mechanism of preventing and curing the Rabbits eyes vitreous hemorrhage in each group． Results：After completion of the model，the vitreous is full of red blood and the eyes fundus cant be visible．Three days later,black turbidness appeared．Observed black floccules in the vitreous during 14 to 30 days and hazed of fundus．The level of MDA in model-control group were significantly higberr than that in normal-control group(p0．01)．Reversely，the activity of SOD were lower(p0．01)．The two prescriptions group were better than the model—control group in decreasing the level MDA．and enhancing the activity of SOD(p0．01)．However，there is no difference in else index between the group of the prescriptions for supplementing Qi to promoting blood circulation and the prescriptions for removing blood stasis by promoting blood circulation groups(p0．05)． Conclusion：The two prescriptions groups can improve the vitreou