环氧合酶-2在重症急性胰腺炎大鼠胰腺组织中表达的实验研究-外科学专业论文.docxVIP

环氧合酶一2在重症急性胰腺炎大鼠胰腺组织中表达的实验研究 环氧合酶一2在重症急性胰腺炎大鼠胰腺组织中 表达的实验研究 摘要 目的 研究环氧合酶一2(COX一2)在重症急性胰腺炎(severe acute pancreatitis，SAP)大鼠胰腺组织中的表达，探讨其在SAP 发病中的可能作用机制。 方法健康成年雄性Sprague—Dawl ey大鼠40只随机分成重症急 性胰腺炎组(SAP，n：20)和正常对照组(NC，n=20)。正常对照组开 腹后只轻轻翻动胰腺后关腹，SAP组大鼠参照Lankishd的方法经胆 胰管恒速逆行注入质量分数为5％的牛磺胆酸钠(1．Oml／kg)制成SAP 大鼠模型。术后12h两组各处死10只大鼠，检测腹水量，血清淀粉 酶(AMS)活性(碘比色法)、肿瘤坏死因子一Q(TNF—a)浓度(ELISA)， 血浆TXB：和6-keto-PGF。。浓度(放免法)，通过光镜观察胰腺的病理 变化，并对胰腺组织进行评分。用免疫组化SABC法观察胰腺组织中 COX一2的表达。两组其余10只大鼠观察24h死亡率及72h存活率。 结果SAP组12h后血清淀粉酶活性、TNF—a浓度，血浆TXB： 和6-keto-PGF。。浓度均显著高于Nc组(P0．01)，SAP组腹水量生 成显著增加(P0．01)。光镜病理评分显示，SAP水肿、炎症、出血 和坏死评分均较Nc组有明显升高(P0．05)。SAP组大鼠胰腺组织 中COX一2蛋白表达为强阳性，而NC组中无COX一2的表达。SAP组大 鼠24h死亡率为100％，NC组死亡率24h为0，48h为10．O％。 结论重症急性胰腺炎时有COX-2的高表达和PGs升高，提示 COX一2可能通过促进PGs升高而在SAP发病中起重要作用，其有可能 成为治疗SAP的作用靶点。 关键词环氧合酶一2，重症急性胰腺炎，前列腺素 Experimental Experimental study on cyclooxygenase一2 expression in Pancreatic tissues of severe acute pancreatitis in rats ABSTRACT objective To study the expression of cyclooxygenase·2(COX一2、 in pancreatic tissues of severe acute pancreatitis(SAP)in rats，and explore the possible role pathogenesis of in SAP． Methods Forty adult male Sprague—Dawley rats were randomly divided into SAP groups(n=20)and control groups(n=20)．Animals in group SAP were induced by the retrograde injection of 5％sodium taurocholate(1．0ml／kg)according to Lankish’S method，and animals in group NC received laparotomy only．At 12h after the induction of SAP, 1 0 rats in each group were sacrificed，acites，serum amylase(AMS) activity was measured by the blue·starch method and serum tumor necrosis factor-a(TNF—a)was determined by ELISA，serum levels of TXB2 and 6-keto—PGFla were detected by radioimmunoassy．The survival time in 72h and mortality in 24h of the rest l 0 rats in SAP and NC group were recorded，and the pathological changes of pancreatic tissues were observed and scored under microscope．Immunohistochemical SABC method was used to detect the expression of cyclooxygenase-2(COX-2) in pancreatic tissue in rats with SAP． R