洛伐他汀对乳腺癌干细胞特性的影响-病理学与病理生理学专业毕业论文.docxVIP

关键词：三阴性乳腺癌；肿瘤干细胞；天然化合物；洛伐他汀III 关键词：三阴性乳腺癌；肿瘤干细胞；天然化合物；洛伐他汀 III 万方数据 AbstractObjeetive： Abstract Objeetive： To study the effects。of Lovastatin(LV)on the biological characteristics of human triple．negative breast cancer stem-like cells(BCSLCs)in vitro，and on xenograft growth of human triple·negative breast cancer cells MDA-MB-23 1 in nude mice． Methods： 1、Breast cancer stem．1ike cells(BCSLCs)were enriched by ultra-low adherence suspension culture from human triple—negative breast cancer cells and non—triple—negative breast cancer cells．The expression of breast cancer stem cell surface markers(CD44／CD24)was detected by flow cytometry．The tumorigenicity of BCSCLs Was confirmed by subcutaneous tumor formation in hude mice． 2、Parental breast cancer cells or BCSLCs were treated with LV or vehicle under normoxia or hypoxia．Cell proliferation，cell migration，and cell apoptosis were detected by MTT assay,scratch assay,and flow cytometry,respectively． 3、MDA．MB．23 1 cells were treated with different concentrations of LV．The protein expression level of breast cancer stem cell surface marker CD44，EMT-related nuclear transcription factor p-catenin，and epithelial cell marker E—cadherin were analyzed by Western blotting． 4、One week after subcutaneous i巧ection of MDA—MB-23 1 cells，the nude mice were treated with different concentrations of LV f2 mg／kg or l Omg／kg)or normal saline，twice weekly for three weeks．The tumor volume Was measured weekly； the xenografl tumors were removed and weighed when the animals were sacrificed．The tumor tissues were embedded in paraffin,and histopathological and immunohistochemical analyses were performed． Results： 1、BCSLCs were enriched from triple—negative and non·triple·negative breast cancer cells using ultra-low adherence suspension culture technique．Cell surface TV 万方数据 markers markers were identified by flow cytometry to obtain purity of about 80％of CD44+／CD24low cells in MDA-MB．．23 1．．derived BCSLCs；in nude mice tumorigenicity experiment，compare