毛细管电色谱研究：填充柱上的场强分布与钛胶基质固定相的研究-药物分析专业毕业论文.docxVIP

Y Y 510527 籀 要 摘要 本文以凝胶诱导聚合法制得了粒径均一的球形氟化钛颗粒，扫描电镜结果 表明，该颗粒粒径为1,09m。并成功的把C18键台到了钛胶表面，制成了十八 烷基铰器定糨，其含酸豢为e。?蚝。懿色谱实验慧袋表臻，芳番纯合饕在这静 固定相上的色谱行为，例如柱效、峰对称性和选择憔，表现不佳，而碱性有机 化合物在其上却能出现较好的色谱峰。 此努，本文还对CEC棱上耱场强分豢送露了繇究。通过实验，本工俸涯交 了在通电的毛细管电色谱柱中，欧姆定律是适用的，也就是说，柱中填充部分 和未填部分的电压降可以用可加合的各段电阻抗与所通过的电流之间的乘积 来摇述。这一点确定惹，爵戮筵荤建逶过采矮溺定遴逶电色漕柱虢窀流，戬及 柱内各段的长度来精确士|虹计算电色谱填充柱上的实际压降。在理论上计算了填 充毛细柱与与其等长空鼍细柱的平均阻抗值之比(趸／冠。)。本文并通过理论 计算，表明一些柱结构参数会对墨，露诋值产生较大影响。作者认为，为了对电 色谱柱中的电渗流现象提供一个合理的浇明，不能以施加电压与电色谱柱长之 比兹方法，麓摹计算乎均场强，夏应渡袋磊奉文爨遴方法对予莠敬±懿电压辍 区分开来精确计算。 关键调：罨绸管电色谱；强定耀；毫凝； 羲辱乏铰； 卡A浣基镟 14：轿脊、释炳阍卷一 黝垒文公布 一一璧塑型———————————————一Abstract 一一璧塑型———————————————一 Abstract Porous titania micro，particles with a very narrow particle size distribution were synthesized by polymerization—induced colloid aggregation(PICA)method·The average diameter of these particles was about 1．0}tm，as measured by scanning electron microscopy(S瓣国。Octadecyl—silyl modified titania was prepared by silylation with octadecyltrichlorosilane．the carbon content was ca．O．7％．However, the experiment results of chromatography show that poor chromatographic behaviors， such as resuming column efficiency,peak symmetry as welt as selectivity of aromatic compounds，when use Octadecyl-silyl modified titania as the stationary phase， whereas basic organic compounds result in relatively good peaks， tn this wo盘the electric field strength distribution over different segments of a packed CEC column was studied On the basis of the experiments，it was proved that Ohm‘s law hetds true in a capillary electrochromatography化EC)column．By using the additivity of the potential drop over the packed and unpacked sections，the exact values ofthe electric field strength over the packed CEC column bed were determined experimentally．The ratio of the resistance of packed CEC column sections to that of an open capillary of the saute length(毪，酝)were calculated．Some theoretical calculations were made to show how some structural parameters of particle lattice affect the resulting弓，‰value of t