蒙药森登4汤化学成分、微量元素及其质量控制方法研究-有机化学专业毕业论文.docxVIP

摘 摘 要 本研究在课题组对森登一4汤不同溶剂提取物的抗炎镇痛药效的研究实验基 础上，对森登一4汤水煎剂的『F丁醇萃取物进行提取、分离、纯化及结构测定， 从中鉴定出3个化合物。它们分别为：槲皮素、2Q，3B．双氢杨梅树皮素和2Q，3p． 双氢杨梅树皮素--4-甲氧基一3，5．二羟基苯甲酸酯。采用微波消解——火焰原子吸 收光度法测定药物中五种微量元素含量，从中初步探讨药物抗风湿药效与微量元 素之间的关系。采用高效液相色谱法对森登一4汤中绿原酸和槲皮素的含量进行 同时测定，所建立的含量测定方法简便、快速，并具有较高的准确度和精密度， 为森登一4汤的质量控制提供依据。 关键词：森登一4汤；化学成分；微量元素；质量控制 Study Study on the Chemical Constituents，Trace Elements in Mongolian Medicine Sendeng一4 Decoction and Its Quality Control Method Abstract Based on the previous comparative study on the anti-inflammatory and analgesic effectsof of different extraction of Sendeng一4 decoction，n-butano Was selected as the extraction solvent．The separation,purification and identification of the compounds from effective part of n·butanol extraction of sendeng-4 decoction were carried out, and three compounds such aS quercetin ，2a，3 p—dihydromyricetin and 2Q，3p-dihydromyricetin-4·methoxy-3，5·dihydroxy benzoate were identified．The determination method of five trace elements by microwave digestionmflame atomic absorption Spectroscopy was developed and the relationship among the anti·rheumatoid effect of the preparation and the contents of five trace elements (K，Ca,Zn，Mn，Fe)in Sendeng-4 Decoction Was discussed．Quercetin and Chlorogenic acid were determined by HPLC in Sendeng一4 Decoction．The methods were simple and accurate and could be used as the quality control methods of Sendeng·4 Decoction． Key wo r d s：Sendeng-4 Decoction：Chemical constituents：Trace Elements?Quali．ty Control Method Di rected by：prof．XU Liang App I i cant for Master degree：k I U Yaj i ng (Organic chemistry) (College ofChemistry，Inner Mongolia University For The Nationalities，Tongliao 028000，China) 插图和附表清单1．表l致小鼠耳肿胀作用的比较 插图和附表清单 1．表l致小鼠耳肿胀作用的比较 ． ．．10 2．表2致小鼠扭体反应效果的比较 ． ．．10 3．表3回归方程及相关系数 ．14 4．表-4原子吸收光谱法操作条件 15 5．表5森登一4汤中微量元素的测定结果 ． ．．15 6．表6绿原酸和槲皮素同收率 ．．18 7．表7样品含量测定 ． 18 8．图1森登一4汤止-J．醇提取物抗风湿活性成分提取分离流程图 8 9．图2森登一4汤样品图 ．．16 10．图3绿原酸、槲皮素对照品图 。 ． ． ．．16 11．附图l 化合物I(槲皮素)12C一ⅦR谱． 23 12．附图2 化合物I(槲