铝诱导不同耐铝型速生桉无性系有机酸分泌及其代谢调控-生态学专业毕业论文.docxVIP

IV IV （4）铝环境下，G9 具有更强的外部排斥铝功能，其根系比 W4 富集 Al 多，但是茎、叶 Al 含量比 W4 少，且 G9 叶片吸收钙能力比 W4 强。 （5）在有机酸代谢酶水平调控上，G9 表现为降低苹果酸的降解酶活 性，提高合成柠檬酸的酶活性以适应 Al 环境，W4 则是合成苹果酸、柠檬 酸相关酶活性不变，显著降低其降解酶水平以适应铝毒害。G9 有机酸酶有 较强的酶调节代谢功能，具备较完善的耐铝机制。 关键词：速生桉；铝；有机酸；有机酸酶；钙 PAGE PAGE VI THE CORRESPOND REGULATION ON AL-INDUCED EXUDATION AND METABOLISM OF ORGANIC ACIDS OF AL-RESISTANCE OF FAST-GROWING IN DIFFERENT ALUMINUM-RESISTANT TYPES OF EUCALYPTUS CLONES ABSTRACT Accumulation of active Al in acidic soil is a limiting factor during plant growth. Eucalyptus is an main timber species in southern China. To study Al-resistance regulation of fast-growing eucalyptus would provide the significant guidance for the sustainable development of fast-growing eucalyptus plantation. The Al-resistance regulation differences of two fast-growing eucalyptus clones G9(Eucalyptus grandis×E.urophylla No.9) and W4(Eucalyptus urophylla No.4) that differing in Al resistance were studied in this research. In different organs, a novel metabolic of Al-induced contents and relative enzyme activities of organic acids and the transport of calcium and aluminum in plant, were researched under hydroponic conditions. Providing the theories for the further study on AI-resistance regulation of fast-growing eucalyptus. The results were as follows: The organic acid exudation of Al-induced from G9 and W4 contains acetate, formate, malate, tartrate, oxalate, citrate, Cis-Aconitrate is another kind of organic acid was found in root apical and leaf. Exudation pattern II for citrate from root apical is the mainly Al-resistance regulation of fast-growing eucalyptus. The G9 clone via secretion pattern I for oxalate, citrate at the beginning of Al-induced treatment. Efflux of citrate and malate increased significantly after 6h (the content of malate account for a rather small proportion), with a marked lag phase between the addition of Al and the onset of organic acid release. The action of protein synthesis related to the metabolism and secretion