角膜内皮细胞相关的部分研究进展和展望.docVIP

角膜内皮细胞相关的部分研究进展和展望 周洪伟(衽平县人民医院252100) 【摘 要】目前己知的角膜内皮损伤理论尚未涉及穿透性角膜移植术后准分子 激光矫正屈光不正对角膜内皮的影响。体外培养人角膜内皮细胞己经比较成熟、 稳定，但仍需进行探索，特别是一些细胞因子和低分子量透明质酸等因素对其的 影响和机制，以更加稳定的体外培养角膜内皮细胞为基础，由数量有限的自体角 膜内皮细胞体外培养构建组织工程角膜将更加便捷。将体外培养自体角膜内皮细 胞来源的组织工稈角膜内皮移植入体内将会较异体角膜内皮移植、异种角膜内皮 移植有更多的优势。异种动物的Descemet膜己被用于动物实验，并且有一定效 果。将异种动物Descemet膜或人工羊膜结合自体角膜内皮细胞来源的组织工程 化角膜内皮进行人类角膜内皮移植，将完全摆脱对人类供体角膜的依赖，或有助 于修复损伤的库存角膜。首先进行眼前节器官培养实验，后序可进行动物实验及 可能的临床实验。 【关键词】角膜内皮细胞；细胞培养；组织工程 Some advances and prospects of the researches related to corneal endothelial cells? ZHOU Hong—wei. Chiping County Peoples Hospital, 252100 China. [Abstract] To our known ledge,the temporal theory about the damage of huma n corneal endothelial cells (HCECs) dont cover the effect of application of excimer laser to treat refractive error which occur after penetrating keratoplasty.The culture of HCECs in vitro is already relatively mature and stable,but it is far cry from a formulized one,and thus deserve more intensive researches.Especially,the effects of some cytokines and hyluronan oligosaccharides on the in vitro culture of HCECs need to be explored as well as the mechanisms behind these effects.And it is eagerly and widely expected that a formulized culture of the HCECs can be hammered out.Base on the proved and more adva need culture formulati on ,to con struct engin eered HCECs from autologous source which is retricted in cells number should be more convenient」f the clinical application of engineered HCECs from autologous source can be fulfilled,more superiorities could be discovered logically compared to the clinical applications of allogeneic HCECs and heterologous HCECs. Descemet membrane lack cell components,and heterologous animals1 decemet membranes are already applied in animal experiments with a certain degree of effectiveness.The combined application of heterologous Decemet membrane or artificial amniotic membrane with engineered HCECs from autologous source in human corneal en dothelium tra nspla ntati on will disengage the res