山豆根多糖的提取工艺及抗肿瘤活性分析-化学工艺专业论文.docxVIP

赣南师 赣南师范学院硕士学位论文 PAGE PAGE 3 Abstracts Radix sophorae tonkinensis is the roots and stem of Leguminous plants of the genus tree in Vietnam Sophora japonica. It is widely recognized that Sophora subprostrate plays multiple roles in pharmacological and biological function as a traditional Chinese herbal medicine, and its has certain antitumor activity. The Masters thesis was carried out to extract and separate polysaccharides from Sophora subprostrate, optimize the extractive technology and study the antitumor activity and its mechanism of through the experiment of inhibiting tumor in vitro directly lethal effect to tumor cell. The thesis include four chapters and each chapter follows: The first chapter: Literature Review. Polysaccharides from sohpara subprastrat. are the main supporting matrix of the edible sohpara subprastrat. and key bioactive components. They have a number of bioactivities such as immunomodulation, anticancer and autoxidation. This paper overviews the extraction technology, the methods of isolation and purification, the chemical structure and the physiological activity in the polysaccharides from the roots of Sophora subprostrata. . The paper also summarized the research results of animal test in recent year and prospected the main research of the polysaccharides form sohpara subprastrat.. The second chapter: The extraction and separation of polysaccharides from sohpara subprastrat. This chapter optimizes the extraction technology of polysaccharides from Sophora Tonkinensis Gapnep. Orthogonal experimental design was used to select optimum technology of Polysaccharides extraction from Sophora Tonkinensis Gapnep. and the content of total polysaccharides were determinated by Phenol-vitriolic Colorimetry. Optional extraction technology of Polysaccharide was as follow: Solid-liquid ratio 1:10; extraction time 90 min;extraction two times . The content of polysaccharide is 3.62%. The method was simple accurate sensitive and suitable for extraction poly