生殖生物学理论与相关技术- 胚胎干细胞.ppt

Tetraploid Blastocyst Complementation From Shaorong Gao in Tongji University Oct4- and Nanog-selection; Viable chimaeras; Contribute to germ line; Generate live late-term embryos when injected into tetraploid blastocysts; The biological potency and epigenetic state are indistinguishable from those of ES cells The second generation of mouse iPS cells Nature 2007 July Chimeric male X C57BL/6 female Tumor formation by c-Myc reactivation 121 F1 mice (8-41 weeks) from Nanog-iPS 20D17 cell line; 24 died or were killed due to illness; 13 mice identified neck tumors, 5 mice with other tu