丙酮酸的测定.pdf

Plant Physiol. (1998) 117: 1083–1093 Analysis of Respiratory Chain Regulation in Roots of Soybean Seedlings1 A. Harvey Millar, Owen K. Atkin, R. Ian Menz, Beverley Henry, Graham Farquhar, and David A. Day* Division of Biochemistry and Molecular Biology, Faculty of Science (A.H.M., R.I.M., D.A.D.), and Environmental Biology Group, Research School of Biological Sciences (O.K.A., B.H., G.F.), The Australian National University, Canberra 0200, Australia tion, specific protein degradation or de novo protein syn- Changes in the respiratory rate and the contribution of the cyto- thesis, and alterations in mitochondrial numbers. chrome (Cyt) c oxidase and alternative oxidase (COX and AOX, The extent to which such changes in respiration rate alter respectively) were investigated in soybean (Glycine max L. cv the rate of oxidative phosphorylation also depends on the Stevens) root seedlings using the 18O-discrimination method. In partitioning of electron flux between the Cyt and the alter- 4-d-old roots respiration proceeded almost entirely via COX, but by native pathways of electron transport. The Cyt pathway d 17 more than 50% of the flux occurred via AOX. During this (terminating at COX) couples the reduction of O2 to water period the capacity of COX, the theoretical yield of ATP synthesis, with the translocation of protons across the inner mito- and the root relative growth rate all decreased substantially. In chondrial membrane, thereby building a proton-motive extracts from whole roots of different ages, the ubiquinone pool wa