膝骨性关节炎关节液分析与相关临床意义的研究.docxVIP

膝骨性关节炎关节液分析与相关临床意义的研究 研究生：谭文成导帅：查振刚 研究生：谭文成 导帅：查振刚 中文摘要 目的：采用多维显微图像分析技术、准弹性激光散射技术检测分析正常膝 关节液与膝骨性关节炎关节液屮颗粒形态结构及其微粒体理化特性，探讨关节液 变化规律，揭示其和互关联变化及有关作用机制。 方法：病例的采集依据临床上诊断标准，抽取正常人和膝骨性关节炎疾病 患者的关节液共99例，采用多维显微图像分析技术、准弹性激光散射技术检测 分析，观察膝骨性关节炎(KOA)早、中、晚期关节液屮颗粒体与正常人关节液 屮颗粒体(直径1 um)的粒度大小与粒度分布状况。用多维显微图像分析技术、 激光散射技术测得的相应不同严重程度KOA疾病的早、屮、晚3个不同吋期关 节颗粒体液微粒的直径数据进行相关性分析。 结果：多维显微成像与分析结果显示，正常人关节液颗粒体的平均接触 面积、周长、长轴、短轴和单位面积(10000 pm2)上的颗粒数分别为69 64呵、2.46 pm、1.50屮n和34个；KOA患者关节液颗粒体的平均接触面积、 周长、长轴、短轴和单位而积(10000 gm2)上的颗粒数分别为97.43 40.70 ym、13.42 pm、8.19 pm和17个，与正常人比较均有显着差异(P0.001)o 激光散射法测得正常关节液的平均粒度分别是63.07nm, KOA的是297.46 nmo早期、屮期、晚期KOA患者关节液屮微粒体的平均粒度分别为239.98nm； 316.83 nm； 345.21mm 统计学分析表明，关节液屮微粒体的大小与KOA的 严重程度密切相关(P0.01,圻0.3642.)。 结论：关节液有形颗粒的形态大小与KOA严重程度密切相关。关节液微粒 休的粒度与KOA、严重程度密切和关。关节液屮微粒体的平均粒度可较准确较快 速地反映关节损伤的程度。 关键词：膝关节骨性关节炎；有形颗粒；微粒体；相关分析 Research the correlation between the analyzing resultof the synovial fluid of knee osteoarthritis and clinicaldiagnosis. Gradute： Tan Wen-cheng Tutor： Zha Zhen-gang Abstract Objective: By using novel multi-dimensional microscopy graphic analysis techinique, Quasi-elasticity laser scattering method, fully analyze the chemical and physical characteristics of micro particles, nano-particles in the knee of synovial fluid of normal and osteoarthritis patients. Investigate the rule of sequence of the change of the synovial fluid, hence promulgate and discover the correlations between the two and its mechanism. Methods: The cases are randomly collected and base on the clinical diagnose standard. Total of 99 specimen of the synovial fluid of the knee are collected from the normal people and those who have been clinically diagnose to have KOA. By using novel multi-dimensional microscopy graphic analysis techinique, quasi-elasticity laser scattering method observe the sizes and distribution of nano-particlcs in the early, mid and final stages of KOA to that of the normal ones (diameterl|.im). Hence from the results we get by using novel multi-dimensional microscopy graphic a