分子病毒学8病毒载体.pptxVIP

-Adenovirus -Retrovirus -Lentivirus -Adeno-associated virus (AAV) -Herpes simplex virus (HSV) ? Non-viral vector based -Naked DNA (plasmid DNA): injection or genegun -Liposomes (cationic lipids): mix with genes ? Ex-vivo ? In vivo;Why vectors __;県..;N^npathogenic human parvovirus, non-enveloped;p aw;Clone Gene off Interest into ITR-Contoining Vector;pAAV-MCS Vector;pUC ori;ampicillin 丨;Verification of Cloning Sequences and binding sites of primers suitable for PCR amplification and/or sequencing applications with pAAV-MCS. pAAV-IRES-hrGFP and pCMV-MCS are shown in the table below.;Generation of adeno-associated virus vector;1-;HEK293 Cells;racteri^cs of AAV vector;Ji后enveloped ds DNA, 36 kilobases;S transgene :;Gutless Adenoviral vector (Amplicon);Bbdjficati^roflhe tropism of;vector;Me;Generation of retroviral vector;Pseudotyped retroviral vector;Icmv/ltrPhP;racteristi;Lentivira;pLP1 Map The figure below shows the features of the pLPl vector. Note that the and pol genes are initially expressed as a gag/pol fusion protein, which is then selfcleaved by the viral protease into individual Gag and Pol polyproteins. The complete sequence of pLPl is available for downloading from our World Wide Web site () or by contacting Technical Service (see page 31).;Features of pLP1;pes simplex virus 1, mild disease in human, no;;ransrernng ora runctionamorma;ppTi c aribfbetge n e;r\r)\;singer;wj!